Status
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Gene information
General information
TC009877 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 12806582..12810805
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC009877
    Gene ontology

    GO terms for Tribolium

    • {{ goTerm.term }}
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      Status: {{ goTerm.status }}
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      Quotation: {{ goTerm.quotation }}
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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
    {{ blurIdResult }}
    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
      {{ unavailableText }}

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_04717

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Adult morphology
    prothoracic odoriferous glands darker - Number of animals showing the phenotype: 7 (13 dpi)
    Days post injection



    abdominal odoriferous glands darker - Number of animals showing the phenotype: 6 (13 dpi)
    Days post injection



    abdominal odoriferous glands content fragmented - Number of animals showing the phenotype: 6 (13 dpi)
    Days post injection



    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): more than 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    eclosion delayed (still pupa) - Number of animals showing the phenotype: 3 (16 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (22 dpi)
    Days post injection


    iBeetle screen iB_04717

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: % (includes death as pupa, adult)

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC009877 RA
      MSLSRKLLV PKRALAVAQ RAYSTDRPY STAIMPELL 
      DELAIPEHV ERVEATPRP YSAIPGPKE LPLIGNAWR 
      FAPIIGQYK IQELDKVMW SLNRDYGRI VKVGGLIGH 
      PDLLFVFNG DDIEKVFRM EEAMPHRPS MPSLHYYKQ 
      ILKKDFFDG NAGVIGVHG PKWEEFRKK VQHALLPPQ 
      IAKKYIEPL DVIAGDFLH RMEDMLDEN QELPNHFLS 
      EIYKWALES VARVSLDTR LGCLEPNLS QNSESQRII 
      NSINTFFWN VAEVELKMP VWRVYKNRS FKKYIGALE 
      DFRTLCLKH IHKSMEKMQ EKNFDEIKE ENISIVERI 
      LLKTDNPKL AAVLALDLL LVGVDTTSI AAASTIYQL 
      SQNPEKQQK LFDELQQVL PENDSKIDV SIQDKMPYL 
      KACIKETLR MYPVIIGNG RSLQTDTVI AGYKVPKGT 
      HVIFPHLVV SNSEDYFHE PHRFLPERW LKTENACPM 
      HKKIHPFVT LPFGYGRRS CLGRRFAEA ELQILLAKI 
      FRKYKVEYN YGPLSYKIT PTYVPEQPL KFKLVKRE
    CDS sequence
    • >TC009877 RA
      atgtccctc tcgcgcaag ttgttggtg cccaaaagg 
      gccctggct gtggcccaa agggcctat tcaaccgat 
      cgtccctac tcgaccgcg atcatgccc gaactgctg 
      gacgagctc gcgattccc gagcacgtg gaacgggtg 
      gaagccacc ccaaggccc tacagcgcg atcccgggc 
      cctaaagag ctgccctta atcggcaac gcctggcgt 
      tttgcgccc atcataggc cagtacaaa atccaggaa 
      ctggataaa gttatgtgg tccctgaac cgggactac 
      ggccggatt gtcaaagtc ggggggctg atagggcac 
      cctgacctt ctgtttgtg ttcaatgga gatgacatc 
      gagaaggtg ttccgcatg gaggaggcc atgccccac 
      aggccctcc atgccctcg ctgcactac tacaagcaa 
      attttgaaa aaggatttt ttcgacggc aacgctgga 
      gtgatcggg gtacatggc cccaagtgg gaggagttc 
      cggaagaag gtccagcac gcgctgctt ccgccccaa 
      attgccaaa aagtacatc gagccgctg gacgtaatt 
      gccggcgat tttcttcac agaatggaa gatatgttg 
      gatgaaaac caggaactg cccaaccac ttcttgtcc 
      gaaatttac aaatgggcc ttggaatct gtggctaga 
      gtctctttg gacactaga cttggctgt ctagaaccg 
      aacctgtcc caaaattcc gaatcacag cgaattatc 
      aattccata aacaccttc ttctggaac gtggcagaa 
      gtagaactg aaaatgccc gtttggagg gtttacaaa 
      aacagaagt ttcaaaaaa tacatcggt gctttggaa 
      gacttccgg acattgtgt ttgaaacac attcataag 
      tcgatggaa aaaatgcaa gagaaaaat tttgacgaa 
      attaaagag gaaaatatc tcgattgtc gaaaggata 
      ttgttgaag acagacaac cccaagttg gctgccgtt 
      ttagccttg gatttgtta ctagtgggg gttgacaca 
      acatcaatc gctgcggcg tccacaatt taccaatta 
      tcccaaaat cccgaaaaa caacaaaaa ctattcgac 
      gaactgcaa caagttttg cccgaaaat gactcaaaa 
      attgacgtc tcgattcag gacaaaatg ccctatctt 
      aaagcctgc attaaagaa acactaaga atgtacccc 
      gtcataata gggaatggg cgaagcctg cagactgac 
      acagtcatc gctggatac aaagttcca aaagggact 
      catgtcatt ttcccgcat ttggttgta agcaacagc 
      gaggactat ttccacgaa ccccatcgt tttctgcca 
      gagaggtgg ctaaagact gaaaatgcc tgtcctatg 
      cataaaaaa attcaccct tttgtgact ctgcccttc 
      ggttatgga cgcagatct tgcctagga agacgattt 
      gcggaagca gagttacaa attttgctg gcaaagatt 
      ttcagaaaa tacaaagtg gaatacaat tacggccct 
      ctttcttac aaaataacg cctacgtat gtacctgaa 
      caaccgctg aagtttaag ctagtgaag cgtgaataa
    mRNA sequence
    • >TC009877 RA
      actcaaaag caaccaccg gcctagtgc tcagaaatg 
      cagttttag tgctgttgc agccgtaag tacatacag 
      tcatgtccc tctcgcgca agttgttgg tgcccaaaa 
      gggccctgg ctgtggccc aaagggcct attcaaccg 
      atcgtccct actcgaccg cgatcatgc ccgaactgc 
      tggacgagc tcgcgattc ccgagcacg tggaacggg 
      tggaagcca ccccaaggc cctacagcg cgatcccgg 
      gccctaaag agctgccct taatcggca acgcctggc 
      gttttgcgc ccatcatag gccagtaca aaatccagg 
      aactggata aagttatgt ggtccctga accgggact 
      acggccgga ttgtcaaag tcggggggc tgatagggc 
      accctgacc ttctgtttg tgttcaatg gagatgaca 
      tcgagaagg tgttccgca tggaggagg ccatgcccc 
      acaggccct ccatgccct cgctgcact actacaagc 
      aaattttga aaaaggatt ttttcgacg gcaacgctg 
      gagtgatcg gggtacatg gccccaagt gggaggagt 
      tccggaaga aggtccagc acgcgctgc ttccgcccc 
      aaattgcca aaaagtaca tcgagccgc tggacgtaa 
      ttgccggcg attttcttc acagaatgg aagatatgt 
      tggatgaaa accaggaac tgcccaacc acttcttgt 
      ccgaaattt acaaatggg ccttggaat ctgtggcta 
      gagtctctt tggacacta gacttggct gtctagaac 
      cgaacctgt cccaaaatt ccgaatcac agcgaatta 
      tcaattcca taaacacct tcttctgga acgtggcag 
      aagtagaac tgaaaatgc ccgtttgga gggtttaca 
      aaaacagaa gtttcaaaa aatacatcg gtgctttgg 
      aagacttcc ggacattgt gtttgaaac acattcata 
      agtcgatgg aaaaaatgc aagagaaaa attttgacg 
      aaattaaag aggaaaata tctcgattg tcgaaagga 
      tattgttga agacagaca accccaagt tggctgccg 
      ttttagcct tggatttgt tactagtgg gggttgaca 
      caacatcaa tcgctgcgg cgtccacaa tttaccaat 
      tatcccaaa atcccgaaa aacaacaaa aactattcg 
      acgaactgc aacaagttt tgcccgaaa atgactcaa 
      aaattgacg tctcgattc aggacaaaa tgccctatc 
      ttaaagcct gcattaaag aaacactaa gaatgtacc 
      ccgtcataa tagggaatg ggcgaagcc tgcagactg 
      acacagtca tcgctggat acaaagttc caaaaggga 
      ctcatgtca ttttcccgc atttggttg taagcaaca 
      gcgaggact atttccacg aaccccatc gttttctgc 
      cagagaggt ggctaaaga ctgaaaatg cctgtccta 
      tgcataaaa aaattcacc cttttgtga ctctgccct 
      tcggttatg gacgcagat cttgcctag gaagacgat 
      ttgcggaag cagagttac aaattttgc tggcaaaga 
      ttttcagaa aatacaaag tggaataca attacggcc 
      ctctttctt acaaaataa cgcctacgt atgtacctg 
      aacaaccgc tgaagttta agctagtga agcgtgaat 
      aagagctta attattgtt tattgttaa acgacttaa 
      gtattatag tgttgtgat gtttttata gaaataata 
      aatctcaat ataacca

    If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
    Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.

    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY