Status
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Gene information
General information
TC008260 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 7466564..7471453
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC008260
    Gene ontology

    GO terms for Tribolium

    • {{ goTerm.term }}
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      Status: {{ goTerm.status }}
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      Quotation: {{ goTerm.quotation }}
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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
    {{ blurIdResult }}
    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
      {{ unavailableText }}

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_04452

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue potentially present, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    larval musculature irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
    cuticle remnants without visible segmentation, differentiation or axis - Penetrance (percentage of animals/eggs on slide): less than 30%
    cuticle everted (inside-out) - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 60.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 4 (16 dpi)
    Days post injection



    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 4 (19 dpi)
    Days post injection


    Adult morphology
    elytra not closed - Number of animals showing the phenotype: 4


    elytra surface irregular - Number of animals showing the phenotype: 2

    iBeetle screen iB_04452

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: % (includes death as pupa, adult)

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC008260 RA
      MASPTPSIE SLPRANSIG SFGDQADYL SLSPLEDSP 
      LSVSGSSPP VSDSVDRDS DPSELHCQQ CREPVADPK 
      LLACFHTFC NPCLEKNKL ICPRCNSES IEGILNSLL 
      FSSDSTTDS FHPTVRCTG CKTKKLDAV ARCVDCANY 
      LCPNCVMAH QFMHCFEGH RVVNLNEIK DESKNGLIS 
      NSTITNGEN KNTVCPRHK GELLKYFCR TCSIPICKE 
      CLNLEHPAG IHEYEHISE AAPKQVEAI QHAVQEAKT 
      KATDIRNIL KNVEHVSSR LQVQYHKAQ NEINDTFMF 
      YRSMLEERK QELLKELES VFSAKQISL GVATQKGQE 
      TVEQIYKTC EFVERLNKC ATIVEILMI RKLLDTKLQ 
      GLLSYNIDQ SVQSACELE FVSNYQAIQ VGVRNTFGY 
      VRSNSEAVV GPSKQPPIA RPTGGSSSS GSSVNGSSG 
      SLNGGIHCP FTISGSSQS TSPFESNII SKRFSSANS 
      LGPFSTTIG DLNLNGINP YEKWSNGGT DTMFQNGSS 
      DPYSLTSTG HTDPMLDLT SKLMSTAIF PPKSQIKRQ 
      KMIYHCKFG EFGVMEGQF TEPSGVAVN AQNDIIVAD 
      TNNHRIQIF DKEGRFKFQ FGECGKRDG QLLYPNRVA 
      VVRTSGDII VTERSPTHQ IQIYNQYGQ FVRKFGANI 
      LQHPRGVTV DNKGRIVVV ECKVMRVII FDQTGTVLQ 
      KFGCSKHLE FPNGVVVND KQEIFISDN RAHCVKVFS 
      YEGVYLRQI GGEGITNYP IGVGINANG EILIADNHN 
      NFNLTIFTQ DGQLVSALE SKVKHAQCF DVALMDDGS 
      VVLASKDYR LYIYRYVQV PPIGL
    CDS sequence
    • >TC008260 RA
      atggcatct ccgactcct tcaattgaa tcactacca 
      cgagcaaac tccattgga tcgtttggt gaccaagca 
      gactacttg tccctgtcc cccttggaa gactcccca 
      ttgtcagtg agcggttcg tcgccccca gtcagcgat 
      tccgtcgat cgagacagc gacccctcc gagctccat 
      tgccagcag tgccgcgag cccgtggcc gaccccaaa 
      ctgttggcc tgcttccac accttctgc aacccttgt 
      ctcgaaaag aacaagctc atctgtccc cggtgcaac 
      tcggaatcg atcgaaggc atcctcaac agcttgctg 
      tttagctcg gactcgacg acagactcg ttccatccg 
      acggtccgt tgcaccggc tgtaagacc aagaaacta 
      gacgcggtt gctcgctgc gtcgattgt gccaattat 
      ctgtgtccg aactgcgtc atggctcat cagttcatg 
      cactgcttc gaaggccat cgagtcgtc aacctgaac 
      gaaatcaaa gatgaatcc aagaacggc ctgatctcg 
      aattccaca atcaccaat ggtgaaaat aagaatacg 
      gtctgtcca cgacacaag ggcgaactt ctcaaatac 
      ttttgtcgc acttgttcg attcccatt tgcaaagag 
      tgtctaaac ctagagcat ccagccggt atacacgaa 
      tacgaacat atcagcgaa gcggcccca aaacaagtc 
      gaagcgatc caacatgcg gttcaagag gctaagacc 
      aaagccacc gacatacgc aacattctc aaaaatgtc 
      gaacacgta tccagtcgc ctgcaggtg caataccac 
      aaagcccaa aacgaaatc aacgacacg ttcatgttc 
      taccgatcg atgctggag gagcggaag caggaactg 
      ttgaaagaa ctagagagc gtattttcg gcaaaacaa 
      atctctctt ggcgtagca acacagaaa ggtcaagaa 
      acggtcgaa cagatttac aaaacgtgc gaattcgtc 
      gagagactg aacaaatgt gctaccatt gttgaaatt 
      ttaatgatt aggaaattg ttagatacg aaattgcaa 
      ggccttttg agttacaac atagatcag agcgtgcaa 
      tcagcatgc gaactggaa ttcgtctcg aattatcag 
      gcgatccaa gtgggcgtc cgtaacacg tttggctac 
      gtccgatcg aattccgaa gccgtggtg ggacccagc 
      aaacaacca cccatcgcc cgtcccacc ggcggctcc 
      agctccagc ggcagcagc gtcaacggt agttcggga 
      agtttaaac ggcggcatc cactgtccc ttcaccatt 
      tccggctcg agccaatca acctctcct ttcgaatcg 
      aacataatc agcaaaaga ttcagcagt gccaatagt 
      ttggggccg ttttcaacg acaatcggt gatttgaat 
      ctcaacggt atcaaccct tacgagaaa tggtcgaac 
      ggtggtacc gacacaatg ttccaaaac ggtagcagc 
      gatccctat tcgttaacg agcaccggt cacaccgac 
      cccatgctc gatctcaca tcgaagttg atgtcgacg 
      gcgatattc cccccgaaa tctcaaatc aaacgccaa 
      aagatgatc taccattgc aagttcggc gagtttggc 
      gtaatggaa ggccagttt actgagccg agcggtgtt 
      gcggtcaac gctcaaaac gacatcatc gtcgccgac 
      acgaacaat catcgaatc cagatcttt gacaaagag 
      ggccgcttc aagttccaa ttcggcgag tgcgggaaa 
      cgcgacggc cagctcctg tatccgaat cgcgtcgct 
      gtcgtgcgt acctcaggc gatatcatc gtcacggag 
      cgttcgccc acgcaccag atccaaatc tacaaccaa 
      tacggacag tttgtgcga aagttcggc gccaacatc 
      ctgcagcat ccgcgcggc gtcaccgtc gacaacaaa 
      ggccgcatc gtcgtcgtc gagtgcaaa gtgatgcgt 
      gtgatcatt ttcgaccaa accgggacc gtgctgcaa 
      aaattcggc tgttcaaag cacttggag ttcccgaac 
      ggtgtcgtc gtcaacgac aagcaggag attttcatc 
      agcgataat cgtgctcac tgcgtcaag gtgttcagt 
      tacgagggt gtttatctg cgacaaatc ggaggcgaa 
      ggcatcacc aactatccg attggtgtc ggtatcaat 
      gccaatggc gagattctg atcgccgat aaccacaac 
      aacttcaac ttgaccatc ttcacccaa gacggacag 
      ttggtgtcc gcgttagag agcaaagtg aagcacgcc 
      caatgtttc gacgtcgca ttgatggac gacggctcc 
      gtagtattg gccagcaag gactaccgt ctgtacatc 
      tatcgctat gtgcaggtg ccccccatt ggcctgtag
    mRNA sequence
    • >TC008260 RA
      ttgttaata aagtggatg acgggtaat taaagatgg 
      catctccga ctccttcaa ttgaatcac taccacgag 
      caaactcca ttggatcgt ttggtgacc aagcagact 
      acttgtccc tgtccccct tggaagact ccccattgt 
      cagtgagcg gttcgtcgc ccccagtca gcgattccg 
      tcgatcgag acagcgacc cctccgagc tccattgcc 
      agcagtgcc gcgagcccg tggccgacc ccaaactgt 
      tggcctgct tccacacct tctgcaacc cttgtctcg 
      aaaagaaca agctcatct gtccccggt gcaactcgg 
      aatcgatcg aaggcatcc tcaacagct tgctgttta 
      gctcggact cgacgacag actcgttcc atccgacgg 
      tccgttgca ccggctgta agaccaaga aactagacg 
      cggttgctc gctgcgtcg attgtgcca attatctgt 
      gtccgaact gcgtcatgg ctcatcagt tcatgcact 
      gcttcgaag gccatcgag tcgtcaacc tgaacgaaa 
      tcaaagatg aatccaaga acggcctga tctcgaatt 
      ccacaatca ccaatggtg aaaataaga atacggtct 
      gtccacgac acaagggcg aacttctca aatactttt 
      gtcgcactt gttcgattc ccatttgca aagagtgtc 
      taaacctag agcatccag ccggtatac acgaatacg 
      aacatatca gcgaagcgg ccccaaaac aagtcgaag 
      cgatccaac atgcggttc aagaggcta agaccaaag 
      ccaccgaca tacgcaaca ttctcaaaa atgtcgaac 
      acgtatcca gtcgcctgc aggtgcaat accacaaag 
      cccaaaacg aaatcaacg acacgttca tgttctacc 
      gatcgatgc tggaggagc ggaagcagg aactgttga 
      aagaactag agagcgtat tttcggcaa aacaaatct 
      ctcttggcg tagcaacac agaaaggtc aagaaacgg 
      tcgaacaga tttacaaaa cgtgcgaat tcgtcgaga 
      gactgaaca aatgtgcta ccattgttg aaattttaa 
      tgattagga aattgttag atacgaaat tgcaaggcc 
      ttttgagtt acaacatag atcagagcg tgcaatcag 
      catgcgaac tggaattcg tctcgaatt atcaggcga 
      tccaagtgg gcgtccgta acacgtttg gctacgtcc 
      gatcgaatt ccgaagccg tggtgggac ccagcaaac 
      aaccaccca tcgcccgtc ccaccggcg gctccagct 
      ccagcggca gcagcgtca acggtagtt cgggaagtt 
      taaacggcg gcatccact gtcccttca ccatttccg 
      gctcgagcc aatcaacct ctcctttcg aatcgaaca 
      taatcagca aaagattca gcagtgcca atagtttgg 
      ggccgtttt caacgacaa tcggtgatt tgaatctca 
      acggtatca acccttacg agaaatggt cgaacggtg 
      gtaccgaca caatgttcc aaaacggta gcagcgatc 
      cctattcgt taacgagca ccggtcaca ccgacccca 
      tgctcgatc tcacatcga agttgatgt cgacggcga 
      tattccccc cgaaatctc aaatcaaac gccaaaaga 
      tgatctacc attgcaagt tcggcgagt ttggcgtaa 
      tggaaggcc agtttactg agccgagcg gtgttgcgg 
      tcaacgctc aaaacgaca tcatcgtcg ccgacacga 
      acaatcatc gaatccaga tctttgaca aagagggcc 
      gcttcaagt tccaattcg gcgagtgcg ggaaacgcg 
      acggccagc tcctgtatc cgaatcgcg tcgctgtcg 
      tgcgtacct caggcgata tcatcgtca cggagcgtt 
      cgcccacgc accagatcc aaatctaca accaatacg 
      gacagtttg tgcgaaagt tcggcgcca acatcctgc 
      agcatccgc gcggcgtca ccgtcgaca acaaaggcc 
      gcatcgtcg tcgtcgagt gcaaagtga tgcgtgtga 
      tcattttcg accaaaccg ggaccgtgc tgcaaaaat 
      tcggctgtt caaagcact tggagttcc cgaacggtg 
      tcgtcgtca acgacaagc aggagattt tcatcagcg 
      ataatcgtg ctcactgcg tcaaggtgt tcagttacg 
      agggtgttt atctgcgac aaatcggag gcgaaggca 
      tcaccaact atccgattg gtgtcggta tcaatgcca 
      atggcgaga ttctgatcg ccgataacc acaacaact 
      tcaacttga ccatcttca cccaagacg gacagttgg 
      tgtccgcgt tagagagca aagtgaagc acgcccaat 
      gtttcgacg tcgcattga tggacgacg gctccgtag 
      tattggcca gcaaggact accgtctgt acatctatc 
      gctatgtgc aggtgcccc ccattggcc tgtagacgg 
      agcgagatc gtcacgaga cagggtctc cagcagccg 
      ttcccgaca ctttttgtt gatgcattt ttgaacgaa 
      gcgttcgga ttgctggtc tgttattac tatttgtat 
      ttatcaatt tataaattt aaagtttgt ccaaaattg 
      taacgatgt gcagtcgtc gtcatagtt ttgtattgt 
      acttcacaa tgttgatat tttttattg atagtatgt 
      acgatattt gacgatgcg tttgagaca ggaagcctg 
      cctgctcct ccaggggag cgcggcccg tcacccgtg 
      ttgaatgac caattctat cactaattc aaaacaggg 
      atattataa ttcacattt tagaaatgt taaagaata 
      ataaaccaa gcagcatag cggtgcctg ggccattgt 
      tgtatttaa gtcgagcat caatttccg atacgatat 
      gatgatcta ttttaatat gcgtgtatg atgttgtgt 
      atgtcctta gtatagcgc gttgttgtt tgcgggatg 
      ccgcgttgt tagcgaccc tagtcaccc acgaactgt 
      acttgtaac agttttttt cttataata ttattggtt 
      atttttaat tatatcccc tccttttcg tatgtattt 
      ttttgtatt attattatt attgtcttt tactactac 
      cgtgctttg ttcgacacc gtagcgtgc cgtgccccc 
      aacttccag tcgctcggg cgttgttta tttttagtt 
      ttgttatga tttataaat agtggtatt attacacca 
      tatctctta agttagaaa aactgcata ggaagaaat 
      tgcaactct ccgtgtggt gcatgcaat atacttcac 
      accatcctt ctttatttt ttttaatta ttatatgag 
      tttagttgt agcagtaga atatatttg tttgttgat 
      gctgccgag tttaagata gtgacgctg gccgcacga 
      gcatgcgtg cggtcgcga aacgcggac gctgccgcc 
      gccgcaggc gcgccctac aagactatt tacttagta 
      gctagggtt ttattttag ttagaaatg gtgagggcg 
      agcgggcgg atggcgtcg ccgtcgtca tcgccgtcg 
      tattcttct catattttt ctctttctc tctattttc 
      gatcgcgat tcccctcgc ccgctccgc ctcgttccg 
      tattattat ttatattat tattatatt gttgttttt 
      tgaaaccgg aggcgatga tcgcagaac acgggggct 
      gagcgatct caatcccac attttacca gcagtcccg 
      tgacttgcg ccgcgccgt cactcgtct ggtcgccga 
      aatgttgtc tcttggtgt ctcaccgac aagtgttat 
      atatgttgt ttttaatgt cttgttcag agtacgctc 
      tattttttc tattgttcc tgagacgtc ctcggtgag 
      gcacctcga gaggagccg gcgcggtcg acccgacca 
      ggatggcta cggcgcccc aatgtatta ctagaattt 
      ttttaaact tgttgttat tattattat cttaatttt 
      aatggtaaa attgttctt aaattaaat gtacattgt 
      tttgttttc tttgatttt ttgttgtaa atgcttttt 
      aattaataa taccaattc ttgcag

    If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
    Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.

    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY