| General information | |||
| TC007431 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007419.2: 4611999..4628810 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC007431 | |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
| Gene | {{ goGene }} |
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| GO ID | * {{ blurIdResult }} |
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| Evidence | {{ blurEvidenceResult }} |
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| Reference | * {{ blurReferenceResult }} |
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| Quotation | ||
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| Publish name | ||
| {{ unavailableText }} |
* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_01186 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)
adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)
- >TC007431 RA
MGHKAMGAI VALTCVLCY YNSCYCGFV FDDISAIKD NRDLRPHSP LLNIFFNDF WGTPMHKEQ SHKSYRPLC VLTFRWNYL LWQLEPMGY HLVNMLLHS VVCLMYFRM CSMFLPELS SFVAAMLFA VHPIHTEAV TGVVGRAET LSSVFFLAA FMLYAKASK SKKSTGWKY LSFSMLSIA TAMLCKEQG ITVAGVCAA YEIFVTQKV RLPEIKHVV KAAITAKSS YHLPWSSEA TKRLLVLSA TTMCLLLAR LQIMGSQLP VFTRFDNPA SVAPTPARQ LTYHYLISV NLWLLLFPC NLCCDWTMG TIPLVETFA DGRNLATIA AYTFFTALL VTAYTTENR QQRTVILMS LALMALPFM PASNLFFPV GFVIAERVL YMPSMGFCM LVAYGWTVL AEKKGKKLA FFLLSILVV THGTKTYLR NWDWEDEYS IFMSGLRVN QKNAKLFNN VGHALESQG KYEEALKFF KTAVSVQED DVGAHINVG RTYNHLKMF KEAEEAYLR AKSLLPKAK PGESYQARI APNHLNVFL NLANLISKN STRLEEADM LYRQAISMR ADYTQAYIN RGDILIKLN RTKEAQEVY ERALLYDSN NPDIYYNLG VVFLEQGKA SQALAYLDK ALEFDPEHE QALLNSAIL LQEFNRPEL RKIARERLL KLLDKDSNN ERVHFNLGM LAMDEKNIE EAEHWFRRA VHLKADFRS SLFNLALLL ADDQRPLEA APFLNQLVK YHPDHVKGL ILLGDIYIN NIKDLDAAE NCYRRILEL DPENIQGLH NLCVVHVER GKLLEAQAC LEKAHQLAP GEDYVLKHL QIVKNRIAK MKLNPDEVE TEKSRSQVV SNKSIDESD RKTRPVVTG DSEEQRYSS RVVNTEPMF VKNVDNIDY VDEDLGEVE FEEGMEQNV SNKWRHGKV GTDNDDPSS GMS
- >TC007431 RA
atggggcac aaggcgatg ggggccatc gtggccctc acctgcgtc ctctgctac tacaacagc tgctactgc gggttcgtc ttcgacgac atcagtgcc atcaaagac aacagagat ctgaggccg cattcgccg ctgcttaac atttttttc aacgacttc tggggcacc cccatgcac aaggagcag agccataag tcgtacagg cccttgtgt gttttgact ttccgatgg aactatttg ctttggcag ctggagccc atggggtat catctcgtg aatatgctc ctccattcc gtcgtttgc ctcatgtac ttcaggatg tgctccatg tttctaccg gagctgtca agtttcgtc gcggccatg ttgttcgcc gttcatccc atccacacg gaggcggtg acgggtgtc gtcggaagg gccgagacg ctctcgtcg gtcttcttt ctggccgct tttatgctc tatgccaaa gcgtccaaa agcaaaaaa tccacaggc tggaaatac ctatcattt tccatgctg tcgatcgca acggcgatg ctgtgcaaa gagcaggga atcacagtc gctggcgta tgtgccgct tatgaaata ttcgtgacc cagaaggtg cgtcttcca gagataaaa cacgtcgta aaggcggcc atcacggcc aagtcctcc taccacctg ccttggtcg agcgaggcc accaagagg ctcctggtg ctttcggcg accactatg tgtcttttg ctggccagg ctccagatc atgggctcc cagctgccg gtcttcacc aggtttgat aacccggcc tcggtggca ccaacgccg gccagacaa ctcacctac cactacctg ataagtgtc aatctttgg ctgcttctg ttcccctgc aatctgtgc tgcgactgg accatgggc accatcccc ttggtcgaa accttcgca gatggacgg aatctggcc actattgcc gcctacacc ttcttcacg gcgctgctg gtgaccgcc tacacgacg gaaaaccgc caacagcgc accgtcata cttatgagc ttggcttta atggccctc cccttcatg cctgcatcc aacctcttt ttccccgtc ggctttgta atcgccgaa cgagtgtta tacatgcct tcgatgggg ttctgcatg ctggtggcc tacggatgg accgtcctc gccgagaaa aagggcaaa aagctcgcc ttttttctc ctaagcatc ctggttgtg acccacggt acaaaaacg tacctccgg aactgggac tgggaggac gagtactcc attttcatg tcggggttg cgagtcaat cagaaaaac gccaagctt tttaataac gtggggcac gccctcgag agccaaggc aagtacgag gaggcgtta aagttcttc aaaacggcg gtgagtgtc caggaggac gacgtcgga gcgcatatt aacgtcggg aggacttac aatcatctc aagatgttc aaagaggct gaagaggcc tacctgcgg gccaagtcg ctcttgccc aaagccaag cctggagag agttaccag gcgaggatt gcgcctaat catctcaac gttttcctc aacctggcg aatttaatt tcgaagaat tcgacgaga ctggaagag gccgatatg ctctaccgg caggccatc agtatgagg gctgattac actcaagct tatattaat cgaggcgat attcttatc aagttgaac aggacgaag gaggcgcaa gaggtctac gagagggcc ctgctctac gacagcaac aaccctgat atttactac aacctcggc gtggtgttc ctggagcag ggcaaggcg tcccaagcc ctcgcttac ctggacaaa gctttagaa ttcgatccg gagcacgag caggctctt ttgaattca gcgatcctc cttcaagaa ttcaaccgg cccgaattg cgtaaaatc gcccgcgaa cgtctattg aaacttcta gacaaagac agcaacaac gagcgggtc catttcaat ctcggaatg ctcgcaatg gacgagaaa aatatcgag gaagccgaa cattggttc cggcgggct gtccattta aaagccgat ttcaggagt tcgctcttc aatttagct ttgttactg gccgacgat cagcggccc ctggaagcc gccccgttc ctcaaccag ctcgtcaag tatcatccc gatcacgtg aaaggactc atcctcctg ggggacatt tatatcaat aacatcaag gacttggac gcggccgaa aattgctat cgcaggatt ctcgagctc gacccggag aacatccag ggtttgcac aacttgtgc gtggttcac gtggagcga gggaagctt ctagaggcg caagcgtgt ctggagaag gcgcaccaa ctggctcct ggggaggat tacgtcttg aaacatctc caaatcgtc aagaatcgc atcgcgaaa atgaaactt aatccagac gaagtcgag acggagaaa agccggagt caggttgtt tcaaataaa agtatagac gagagtgat cggaagacg cggcccgtg gtcacggga gactccgag gagcagagg tatagcagt agagtggta aacactgaa cccatgttt gtgaaaaat gttgacaat attgactat gttgatgaa gatttggga gaggtggag ttcgaggaa gggatggag caaaatgtt agtaataag tggaggcat ggtaaggtc ggtactgac aatgatgac ccgtcttca ggcatgtct tag
- >TC007431 RA
gactccgga ggaatccgg tgcgcatat tccagaggg tcacgtgag gcttcgacg cagtatggc agccatcac accagttgg gttcttgcg acttagtgc tttcgaact gttggctgg gtgtggacg tcccctcgt gcgtctgca atgctcaca caactgtta cgtgcaagt ggtgcgagg tgcagtgac ccggagtga tgctccact agcccccag agtgcgaaa gacatgggg cacaaggcg atgggggcc atcgtggcc ctcacctgc gtcctctgc tactacaac agctgctac tgcgggttc gtcttcgac gacatcagt gccatcaaa gacaacaga gatctgagg ccgcattcg ccgctgctt aacattttt ttcaacgac ttctggggc acccccatg cacaaggag cagagccat aagtcgtac aggcccttg tgtgttttg actttccga tggaactat ttgctttgg cagctggag cccatgggg tatcatctc gtgaatatg ctcctccat tccgtcgtt tgcctcatg tacttcagg atgtgctcc atgtttcta ccggagctg tcaagtttc gtcgcggcc atgttgttc gccgttcat cccatccac acggaggcg gtgacgggt gtcgtcgga agggccgag acgctctcg tcggtcttc tttctggcc gcttttatg ctctatgcc aaagcgtcc aaaagcaaa aaatccaca ggctggaaa tacctatca ttttccatg ctgtcgatc gcaacggcg atgctgtgc aaagagcag ggaatcaca gtcgctggc gtatgtgcc gcttatgaa atattcgtg acccagaag gtgcgtctt ccagagata aaacacgtc gtaaaggcg gccatcacg gccaagtcc tcctaccac ctgccttgg tcgagcgag gccaccaag aggctcctg gtgctttcg gcgaccact atgtgtctt ttgctggcc aggctccag atcatgggc tcccagctg ccggtcttc accaggttt gataacccg gcctcggtg gcaccaacg ccggccaga caactcacc taccactac ctgataagt gtcaatctt tggctgctt ctgttcccc tgcaatctg tgctgcgac tggaccatg ggcaccatc cccttggtc gaaaccttc gcagatgga cggaatctg gccactatt gccgcctac accttcttc acggcgctg ctggtgacc gcctacacg acggaaaac cgccaacag cgcaccgtc atacttatg agcttggct ttaatggcc ctccccttc atgcctgca tccaacctc tttttcccc gtcggcttt gtaatcgcc gaacgagtg ttatacatg ccttcgatg gggttctgc atgctggtg gcctacgga tggaccgtc ctcgccgag aaaaagggc aaaaagctc gcctttttt ctcctaagc atcctggtt gtgacccac ggtacaaaa acgtacctc cggaactgg gactgggag gacgagtac tccattttc atgtcgggg ttgcgagtc aatcagaaa aacgccaag ctttttaat aacgtgggg cacgccctc gagagccaa ggcaagtac gaggaggcg ttaaagttc ttcaaaacg gcggtgagt gtccaggag gacgacgtc ggagcgcat attaacgtc gggaggact tacaatcat ctcaagatg ttcaaagag gctgaagag gcctacctg cgggccaag tcgctcttg cccaaagcc aagcctgga gagagttac caggcgagg attgcgcct aatcatctc aacgttttc ctcaacctg gcgaattta atttcgaag aattcgacg agactggaa gaggccgat atgctctac cggcaggcc atcagtatg agggctgat tacactcaa gcttatatt aatcgaggc gatattctt atcaagttg aacaggacg aaggaggcg caagaggtc tacgagagg gccctgctc tacgacagc aacaaccct gatatttac tacaacctc ggcgtggtg ttcctggag cagggcaag gcgtcccaa gccctcgct tacctggac aaagcttta gaattcgat ccggagcac gagcaggct cttttgaat tcagcgatc ctccttcaa gaattcaac cggcccgaa ttgcgtaaa atcgcccgc gaacgtcta ttgaaactt ctagacaaa gacagcaac aacgagcgg gtccatttc aatctcgga atgctcgca atggacgag aaaaatatc gaggaagcc gaacattgg ttccggcgg gctgtccat ttaaaagcc gatttcagg agttcgctc ttcaattta gctttgtta ctggccgac gatcagcgg cccctggaa gccgccccg ttcctcaac cagctcgtc aagtatcat cccgatcac gtgaaagga ctcatcctc ctgggggac atttatatc aataacatc aaggacttg gacgcggcc gaaaattgc tatcgcagg attctcgag ctcgacccg gagaacatc cagggtttg cacaacttg tgcgtggtt cacgtggag cgagggaag cttctagag gcgcaagcg tgtctggag aaggcgcac caactggct cctggggag gattacgtc ttgaaacat ctccaaatc gtcaagaat cgcatcgcg aaaatgaaa cttaatcca gacgaagtc gagacggag aaaagccgg agtcaggtt gtttcaaat aaaagtata gacgagagt gatcggaag acgcggccc gtggtcacg ggagactcc gaggagcag aggtatagc agtagagtg gtaaacact gaacccatg tttgtgaaa aatgttgac aatattgac tatgttgat gaagatttg ggagaggtg gagttcgag gaagggatg gagcaaaat gttagtaat aagtggagg catggtaag gtcggtact gacaatgat gacccgtct tcaggcatg tcttagcat taaaagagt attttcgaa tcaaattaa tgtaatttt aatgagccc ccgtaaggg gttttactc tatctaagg actttttta tacgagcca ttagatgta gtgtccatt ctgtgatac tcagaataa tcgtttttg aatctattt ttgatctgg ttttgttcg atctaagac ctatgcaga tgtgactta atgtataca agattttcg cacttgttt tttgtgatt tttcattgt gattcattt tgtgattag ctttatgta tcgaggtgt tttgtcgct tgttttgct taaatcaaa tgttactta atgtgatta tttgattcc tgtcgattg tttgtcggt aagtggcat atctgtaat atgttaaat tagtaaata ttgctacta atataatca ataaaaata aatgtattg atgtaaggc ttgttagcc gctagtttt agacgacat ccacgtggc caaatattc agtagaaat ccttagcac ttctcaatc atttgtcta ttttttcgg gtgaaaaaa ttgttattt ttgctatcg tggatctta atttatttc gtattttgt aaaggtgcc ttggaatcg ttaattaag gcaaatgat tttgtaata tagtataat aaatatttt tctatgttg tcaaactgt tcatagatt ataaacata agtctatat tgtgtaaaa ctaatgtga gaggtatat ttatcctat gactgataa gttttcgtg tattttctt tcgaaattt tctaaaagt tgttaccct aatatacac ttttttaat aaaaccagt tgtggtat
If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.
