Status
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Gene information
General information
TC007336 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 5989301..5994926
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC007336
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes

    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_01167

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection

    Fertility
    egg chamber mostly not present - Number of animals showing the phenotype: 4

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    eclosion not fulfilled (not completely eclosed) - Number of animals showing the phenotype: 6 (16 dpi)
    Days post injection


    eclosion not fulfilled (not completely eclosed) - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection

    Pupal morphology
    wings slightly shortened -

    wings shape irregular - Number of animals showing the phenotype: 8
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC007336 RA
      MVLAEKNSE NARNGRSRG PSPNGHVQD STSEDESSV 
PVDKIRVGR DYQAICPEL QPESQRKPE LLADRALLV 
WSPTESIPE SKLEDYIVL AKDKYGYNG EQALGMLFW 
HKHDLDRAV LDLANFTPF PDEWSVEDK VLFEQAFQF 
HGKSFHRIR QMLPDKSIA SLVKYYYSW KKTRSRTSL 
MDKQARKLN TPKEEGGAS EAGSEGGSN EESDHDDKI 
QQNKQNISS LKRKHADSI EDLRPTNDN SSRINARWT 
NEELLLAVQ GVRKYGKDF KSIAEVLGN KTEHHVRTF 
FVNYRKRYN LDNVLKDWE KDNGPLPED NGDVKVELD 
DSNNDNDVI CISPTPKKE MKNGKVTQA SK
    • >TC007336 RB
      MVLAEKNSE NARNGRSRG PSPNGHVQD STSEDESSV 
PVDKIRVGR DYQAICPEL QPESQRKPE LLADRALLV 
WSPTESIPE SKLEDYIVL AKDKYGYNG EQALGMLFW 
HKHDLDRAV LDLANFTPF PDEWSVEDK VLFEQAFQF 
HGKSFHRIR QMLPDKSIA SLVKYYYSW KKTRSRTSL 
MDKQARKLN TPKEEGGAS EAGSEGGSN EESDHDDKG 
EGGACSNCG VACTVTQVT SKGSLCNSC YQHWRRTGL 
LRPTSGPTG GKRGSPLGG RHKRKPPRG MYINHDDLA 
AMASGNAGV LMLKAMDRE LDSLQRQIQ QNKQNISSL 
KRKHADSIE DLRPTNDNS SRINARWTN EELLLAVQG 
VRKYGKDFK SIAEVLGNK TEHHVRTFF VNYRKRYNL 
DNVLKDWEK DNGPLPEDN GDVKVELDD SNNDNDVIC 
ISPTPKKEM KNGKVTQAS K
    CDS sequence
    • >TC007336 RA
      atggttcta gccgaaaaa aacagtgaa aatgcccga 
aacggtcgg agtcgagga ccgagtccc aatggtcac 
gttcaagac tccacaagc gaggatgaa agctcagtt 
ccagtagat aagattcga gttgggcga gactaccag 
gccatttgc cccgaactt cagccggaa tcgcagcga 
aaacccgaa ttattggcc gatcgagcg ctacttgta 
tggtcacca actgagtcc atccccgag tcaaagctg 
gaggattac atagtttta gccaaagac aagtacggt 
tacaacggg gagcaggcg cttgggatg ctcttttgg 
cacaagcat gatttagac cgggcggtg ctggacttg 
gcgaacttc acccccttc cccgacgag tggtcggtc 
gaagacaag gtgctgttc gagcaggcg tttcagttc 
cacggcaaa agctttcat aggataaga caaatgttg 
ccagataaa agtattgca agtttagtc aagtattac 
tacagttgg aaaaagaca aggtctagg accagcctc 
atggacaag caggcacgt aagttgaac acccccaag 
gaggaaggg ggcgcttca gaggccggc tcagaggga 
ggatccaac gaggaatca gaccatgac gataagatc 
cagcagaac aagcagaat ataagttcc ttaaagcgc 
aagcatgca gactccatt gaagatctc cgtccaaca 
aatgacaat tcgtctcgt attaacgcc cgctggaca 
aacgaggaa ttgctcttg gctgtccaa ggcgtgcgt 
aagtacggc aaagacttt aaaagcatc gccgaagta 
ttgggtaac aaaactgaa caccatgtg cgtacgttt 
ttcgttaat taccgtaaa agatataat ttagacaat 
gttctaaaa gactgggaa aaagataat gggcctttg 
cctgaagat aacggcgac gttaaagtc gaattggac 
gattccaac aatgataac gatgttatt tgtatatca 
ccgacccca aaaaaagaa atgaaaaac gggaaagtc 
actcaagct agtaaatga
    • >TC007336 RB
      atggttcta gccgaaaaa aacagtgaa aatgcccga 
aacggtcgg agtcgagga ccgagtccc aatggtcac 
gttcaagac tccacaagc gaggatgaa agctcagtt 
ccagtagat aagattcga gttgggcga gactaccag 
gccatttgc cccgaactt cagccggaa tcgcagcga 
aaacccgaa ttattggcc gatcgagcg ctacttgta 
tggtcacca actgagtcc atccccgag tcaaagctg 
gaggattac atagtttta gccaaagac aagtacggt 
tacaacggg gagcaggcg cttgggatg ctcttttgg 
cacaagcat gatttagac cgggcggtg ctggacttg 
gcgaacttc acccccttc cccgacgag tggtcggtc 
gaagacaag gtgctgttc gagcaggcg tttcagttc 
cacggcaaa agctttcat aggataaga caaatgttg 
ccagataaa agtattgca agtttagtc aagtattac 
tacagttgg aaaaagaca aggtctagg accagcctc 
atggacaag caggcacgt aagttgaac acccccaag 
gaggaaggg ggcgcttca gaggccggc tcagaggga 
ggatccaac gaggaatca gaccatgac gataagggt 
gagggtggc gcgtgctct aactgcgga gtcgcctgt 
accgtcact caagtgacg tccaagggg tccctctgt 
aattcctgc taccaacac tggagacga acgggattg 
ttgcggccc acatcgggg cccacgggt ggcaagcgc 
gggtcccca ctgggcgga cgccacaag cgcaagccc 
cctcgcggc atgtacatc aaccacgac gatctggct 
gcgatggcg tcaggcaac gcgggtgtg ctcatgctt 
aaggcgatg gatcgagaa ttagattcc ctccaaaga 
caaatccag cagaacaag cagaatata agttcctta 
aagcgcaag catgcagac tccattgaa gatctccgt 
ccaacaaat gacaattcg tctcgtatt aacgcccgc 
tggacaaac gaggaattg ctcttggct gtccaaggc 
gtgcgtaag tacggcaaa gactttaaa agcatcgcc 
gaagtattg ggtaacaaa actgaacac catgtgcgt 
acgtttttc gttaattac cgtaaaaga tataattta 
gacaatgtt ctaaaagac tgggaaaaa gataatggg 
cctttgcct gaagataac ggcgacgtt aaagtcgaa 
ttggacgat tccaacaat gataacgat gttatttgt 
atatcaccg accccaaaa aaagaaatg aaaaacggg 
aaagtcact caagctagt aaatga
    mRNA sequence
    • >TC007336 RA
      gtggtcgtt gaaaaacgc taaaaatta cgatgtatt 
gtggaaaaa atgcgtttg accgtagcg ccgtggcgg 
tttttggaa ataagccgt ggtaaacta cggttctcc 
gatgacgat tttctagtc gctttgtag tttgagtaa 
gctgtggtg ttgctgaat catagactc ggttttttc 
ttaagatta attttctat tatttatgt gaagctcta 
gttaggagg agcgaagat ggttctagc cgaaaaaaa 
cagtgaaaa tgcccgaaa cggtcggag tcgaggacc 
gagtcccaa tggtcacgt tcaagactc cacaagcga 
ggatgaaag ctcagttcc agtagataa gattcgagt 
tgggcgaga ctaccaggc catttgccc cgaacttca 
gccggaatc gcagcgaaa acccgaatt attggccga 
tcgagcgct acttgtatg gtcaccaac tgagtccat 
ccccgagtc aaagctgga ggattacat agttttagc 
caaagacaa gtacggtta caacgggga gcaggcgct 
tgggatgct cttttggca caagcatga tttagaccg 
ggcggtgct ggacttggc gaacttcac ccccttccc 
cgacgagtg gtcggtcga agacaaggt gctgttcga 
gcaggcgtt tcagttcca cggcaaaag ctttcatag 
gataagaca aatgttgcc agataaaag tattgcaag 
tttagtcaa gtattacta cagttggaa aaagacaag 
gtctaggac cagcctcat ggacaagca ggcacgtaa 
gttgaacac ccccaagga ggaaggggg cgcttcaga 
ggccggctc agagggagg atccaacga ggaatcaga 
ccatgacga taagatcca gcagaacaa gcagaatat 
aagttcctt aaagcgcaa gcatgcaga ctccattga 
agatctccg tccaacaaa tgacaattc gtctcgtat 
taacgcccg ctggacaaa cgaggaatt gctcttggc 
tgtccaagg cgtgcgtaa gtacggcaa agactttaa 
aagcatcgc cgaagtatt gggtaacaa aactgaaca 
ccatgtgcg tacgttttt cgttaatta ccgtaaaag 
atataattt agacaatgt tctaaaaga ctgggaaaa 
agataatgg gcctttgcc tgaagataa cggcgacgt 
taaagtcga attggacga ttccaacaa tgataacga 
tgttatttg tatatcacc gaccccaaa aaaagaaat 
gaaaaacgg gaaagtcac tcaagctag taaatgata 
actttgtac tcgtttctg atttgttgg ataggttcg 
ttttttatt catatcgac gtgtattat gtattgttt 
ttaataaaa taatatatc caac
    • >TC007336 RB
      gtggtcgtt gaaaaacgc taaaaatta cgatgtatt 
gtggaaaaa atgcgtttg accgtagcg ccgtggcgg 
tttttggaa ataagccgt ggtaaacta cggttctcc 
gatgacgat tttctagtc gctttgtag tttgagtaa 
gctgtggtg ttgctgaat catagactc ggttttttc 
ttaagatta attttctat tatttatgt gaagctcta 
gttaggagg agcgaagat ggttctagc cgaaaaaaa 
cagtgaaaa tgcccgaaa cggtcggag tcgaggacc 
gagtcccaa tggtcacgt tcaagactc cacaagcga 
ggatgaaag ctcagttcc agtagataa gattcgagt 
tgggcgaga ctaccaggc catttgccc cgaacttca 
gccggaatc gcagcgaaa acccgaatt attggccga 
tcgagcgct acttgtatg gtcaccaac tgagtccat 
ccccgagtc aaagctgga ggattacat agttttagc 
caaagacaa gtacggtta caacgggga gcaggcgct 
tgggatgct cttttggca caagcatga tttagaccg 
ggcggtgct ggacttggc gaacttcac ccccttccc 
cgacgagtg gtcggtcga agacaaggt gctgttcga 
gcaggcgtt tcagttcca cggcaaaag ctttcatag 
gataagaca aatgttgcc agataaaag tattgcaag 
tttagtcaa gtattacta cagttggaa aaagacaag 
gtctaggac cagcctcat ggacaagca ggcacgtaa 
gttgaacac ccccaagga ggaaggggg cgcttcaga 
ggccggctc agagggagg atccaacga ggaatcaga 
ccatgacga taagggtga gggtggcgc gtgctctaa 
ctgcggagt cgcctgtac cgtcactca agtgacgtc 
caaggggtc cctctgtaa ttcctgcta ccaacactg 
gagacgaac gggattgtt gcggcccac atcggggcc 
cacgggtgg caagcgcgg gtccccact gggcggacg 
ccacaagcg caagccccc tcgcggcat gtacatcaa 
ccacgacga tctggctgc gatggcgtc aggcaacgc 
gggtgtgct catgcttaa ggcgatgga tcgagaatt 
agattccct ccaaagaca aatccagca gaacaagca 
gaatataag ttccttaaa gcgcaagca tgcagactc 
cattgaaga tctccgtcc aacaaatga caattcgtc 
tcgtattaa cgcccgctg gacaaacga ggaattgct 
cttggctgt ccaaggcgt gcgtaagta cggcaaaga 
ctttaaaag catcgccga agtattggg taacaaaac 
tgaacacca tgtgcgtac gtttttcgt taattaccg 
taaaagata taatttaga caatgttct aaaagactg 
ggaaaaaga taatgggcc tttgcctga agataacgg 
cgacgttaa agtcgaatt ggacgattc caacaatga 
taacgatgt tatttgtat atcaccgac cccaaaaaa 
agaaatgaa aaacgggaa agtcactca agctagtaa 
atgataact ttgtactcg tttctgatt tgttggata 
ggttcgttt tttattcat atcgacgtg tattatgta 
ttgttttta ataaaataa tatatccaa c

    If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
    Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.

    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY