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Gene information
General information
TC006819 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 14570639..14571879
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC006819
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_11044

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC006819 RA
      MVVCKYFLQ GTCRFGENC KFEHQISAD HSYAGSQSV 
      LVQNYANKP APQNASTAV DTNTLVKAV VNDMTCAEK 
      GGQWLLSSY SPFRDKPQF PGFEDHSME EIRYLFYES 
      AKNGTIEQF KQNLQMMLQ QAITKIKAL QNPSLDVIN 
      ILKNIYNTP PSNQTQNSI FGGSQMQQK PAFGNTTQP 
      SGIFGGVQT QSVFAANKV PNPIFGEQQ NIQNKSIFA 
      QASTQASNS MFGAPFPQN TTQPQQNIF QQQPPPFVA 
      AQQPPSTSH FGSVNQFTA QQFGDTTSN KPAAFQHTQ 
      SIFGNFGMQ QQEQNVFGG APHQTKPNQ DFYSKLEDL 
      TEEEIKWFQ SDNLDMLKI PEKPPTYEM CFKVQ
    CDS sequence
    • >TC006819 RA
      atggttgtt tgcaagtat tttttgcaa ggtacttgc 
      agatttggt gaaaattgt aaatttgaa catcaaatc 
      agtgcagat cacagttac gccggttct cagtcggtt 
      ttagtccaa aattacgca aacaaacca gcaccccag 
      aatgcgtca actgctgtt gatacaaac actttggtg 
      aaagcagta gtgaatgat atgacttgt gcagaaaag 
      ggtggccaa tggctctta tcgtcatat tccccattt 
      cgtgacaag ccccagttt cccggtttt gaagatcac 
      agtatggaa gaaatcaga tatttattt tacgaatca 
      gccaaaaac ggcactatt gaacaattc aaacaaaat 
      ttgcaaatg atgttacaa caggcaata acgaaaatc 
      aaagccttg caaaacccc tcacttgat gttattaac 
      atactaaaa aatatctac aatactcca ccatcaaat 
      caaacgcaa aattcgatt tttggaggc tcccagatg 
      cagcaaaaa cccgctttt ggtaacaca actcaacca 
      agtggaatt ttcggcggg gttcaaaca cagagtgtg 
      tttgctgct aataaggtc cctaatcca atttttgga 
      gaacaacaa aacattcaa aataaatcg attttcgca 
      caagcctca acacaagcg agtaactca atgtttggg 
      gcaccattt ccgcaaaat acgacgcag ccccaacaa 
      aatattttc caacaacag cctccccct tttgtcgca 
      gcacaacag cccccctca actagtcat tttgggagt 
      gtaaatcaa tttacggca caacagttt ggggataca 
      acgagtaat aaaccggcg gcgtttcaa catactcaa 
      agtattttt ggcaatttt ggaatgcaa cagcaggaa 
      caaaatgtg tttggtggc gcacctcat cagactaaa 
      ccaaatcaa gatttttac tccaagttg gaagatttg 
      actgaagag gagataaag tggttccaa agtgataat 
      ttagacatg ttaaagata ccggaaaaa ccgccaacg 
      tatgaaatg tgcttcaag gtgcaataa
    mRNA sequence
    • >TC006819 RA
      aatttttca gatctaaca attttttcc tctttttgc 
      gatggttgt ttgcaagta ttttttgca aggtacttg 
      cagatttgg tgaaaattg taaatttga acatcaaat 
      cagtgcaga tcacagtta cgccggttc tcagtcggt 
      tttagtcca aaattacgc aaacaaacc agcacccca 
      gaatgcgtc aactgctgt tgatacaaa cactttggt 
      gaaagcagt agtgaatga tatgacttg tgcagaaaa 
      gggtggcca atggctctt atcgtcata ttccccatt 
      tcgtgacaa gccccagtt tcccggttt tgaagatca 
      cagtatgga agaaatcag atatttatt ttacgaatc 
      agccaaaaa cggcactat tgaacaatt caaacaaaa 
      tttgcaaat gatgttaca acaggcaat aacgaaaat 
      caaagcctt gcaaaaccc ctcacttga tgttattaa 
      catactaaa aaatatcta caatactcc accatcaaa 
      tcaaacgca aaattcgat ttttggagg ctcccagat 
      gcagcaaaa acccgcttt tggtaacac aactcaacc 
      aagtggaat tttcggcgg ggttcaaac acagagtgt 
      gtttgctgc taataaggt ccctaatcc aatttttgg 
      agaacaaca aaacattca aaataaatc gattttcgc 
      acaagcctc aacacaagc gagtaactc aatgtttgg 
      ggcaccatt tccgcaaaa tacgacgca gccccaaca 
      aaatatttt ccaacaaca gcctccccc ttttgtcgc 
      agcacaaca gcccccctc aactagtca ttttgggag 
      tgtaaatca atttacggc acaacagtt tggggatac 
      aacgagtaa taaaccggc ggcgtttca acatactca 
      aagtatttt tggcaattt tggaatgca acagcagga 
      acaaaatgt gtttggtgg cgcacctca tcagactaa 
      accaaatca agattttta ctccaagtt ggaagattt 
      gactgaaga ggagataaa gtggttcca aagtgataa 
      tttagacat gttaaagat accggaaaa accgccaac 
      gtatgaaat gtgcttcaa ggtgcaata agttttcaa 
      gtactacga gattttact gtttatttt ttgattgaa 
      taacaaaca taataaatc aattttttt ttt

    If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
    Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.

    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY