Status
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Gene information
General information
TC006109 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 2525028..2532762
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC006109
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes

    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_00997

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): more than 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
     embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC006109 RA
      MSEREDNVY KAKLAEQAE RYDEMVDAM KKVAKLDLE 
LTVEERNLL SVAYKNVIG ARRASWRII SSIEQKEES 
KGTDDKLEM IRQYRSQVE KELRDICSD ILNVLDKHL 
IPAASTGES KVFYYKMKG DYHRYLAEF ATGNDRKDA 
AEHSLVAYK SASDIAMTE LPPTHPIRL GLALNFSVF 
YYEILNSPD RACRLAKAA FDDAIAELD TLSEESYKD 
STLIMQLLR DNLTLWTSD MQGDGEAEP KEQLQDVED 
QDVS
    CDS sequence
    • >TC006109 RA
      atgtctgaa cgggaggat aacgtctac aaggcgaaa 
ttggcagaa caggccgaa cgttacgat gagatggtc 
gacgccatg aagaaggtg gccaagctg gacttggag 
ttgaccgtc gaagagcgc aatcttctg tcggtggct 
tacaagaac gtgatcggt gcccgacga gcgtcctgg 
cgtataata tcgtcaatt gaacagaag gaagaaagc 
aaaggcacc gacgacaaa ttggaaatg ataagacag 
tacaggtca caagtcgag aaagaattg agagacatc 
tgttcggat attttgaac gtattggat aaacatctg 
ataccggcg gcgtcaacc ggcgaatcg aaagtcttc 
tattataaa atgaagggt gattaccat cgatatttg 
gccgagttc gcaacaggc aacgatcgc aaagacgcc 
gccgagcat tcgttggtc gcttacaag tcagcgagc 
gacatcgca atgacggaa ctcccgccc acgcacccg 
attcggctc ggtctggcg ctaaatttc tcggtattt 
tactacgag atattaaat agccccgat agggcctgt 
cgtctggcg aaggcggcc ttcgacgac gccatcgcc 
gagttggac acgttatcc gaagagagc tacaaagac 
agcacgctg atcatgcaa ttgttgcgc gacaacctg 
acgctgtgg accagtgat atgcaagga gacggcgag 
gcggagccc aaagagcag ctgcaggac gtagaggac 
caggacgtg tcgtaa
    mRNA sequence
    • >TC006109 RA
      taggacgca taatttaat tattgtggc atgcagtgt 
gtgaaagat aagcaggat taaataatt atttattca 
atttggcgc cattgtgac gtgggccgg aggcagccg 
atgtgcgtc cgatcgcgt tcgggttta gtgtggcgg 
gcgcggcca tattggcag tacgttgag tgtctgtgt 
tttttagtg ttgtgaaat ttctatcga taatcatcg 
caaaatgtc tgaacggga ggataacgt ctacaaggc 
gaaattggc agaacaggc cgaacgtta cgatgagat 
ggtcgacgc catgaagaa ggtggccaa gctggactt 
ggagttgac cgtcgaaga gcgcaatct tctgtcggt 
ggcttacaa gaacgtgat cggtgcccg acgagcgtc 
ctggcgtat aatatcgtc aattgaaca gaaggaaga 
aagcaaagg caccgacga caaattgga aatgataag 
acagtacag gtcacaagt cgagaaaga attgagaga 
catctgttc ggatatttt gaacgtatt ggataaaca 
tctgatacc ggcggcgtc aaccggcga atcgaaagt 
cttctatta taaaatgaa gggtgatta ccatcgata 
tttggccga gttcgcaac aggcaacga tcgcaaaga 
cgccgccga gcattcgtt ggtcgctta caagtcagc 
gagcgacat cgcaatgac ggaactccc gcccacgca 
cccgattcg gctcggtct ggcgctaaa tttctcggt 
attttacta cgagatatt aaatagccc cgatagggc 
ctgtcgtct ggcgaaggc ggccttcga cgacgccat 
cgccgagtt ggacacgtt atccgaaga gagctacaa 
agacagcac gctgatcat gcaattgtt gcgcgacaa 
cctgacgct gtggaccag tgatatgca aggagacgg 
cgaggcgga gcccaaaga gcagctgca ggacgtaga 
ggaccagga cgtgtcgta actgtttag ctgatctcc 
agcgactac gaactaacc agtgctgtg tagtgcggg 
ccaggccca gacattcga accggaggc ggtaaccgt 
ctcgatccc agtacatac tgtattatt ataattatc 
ttagccgta actctatat tgtatctgt ttgtctcaa 
aatgtgtga atgagagag cctctctac gagcgactg 
ttctctgat ttctctcct ctctccttg ccctgtctc 
ctcggccat tgtcccggc gcacgcaat gttaatctt 
tttctatta aacatcaaa aataaacaa attaaacgt 
actctcggt ctagcagct tggcctccc cctttatac 
tccctccca gtgagagtg gcgtgtggt gcgaatgag 
gcgagtgtc ttgtttatt ttcccgctt tatttatat 
tgagtttga acgaaaaag gcagccttt cgagggaat 
tgtagtatt aggtgattg atttttggc aatagagca 
gtatttttg tggcgagca aaaacacgc atatttccg 
tacaaatgg cattccgct attttcgag tacttacag 
tagatttta tgtgatggt gattgcatg tcgagcaat 
aagtttttt ctattttgt aaatctatt tttttacat 
cactgtttt atcggaaaa ctggtgaaa tttcagttt 
ttcgcggta cttaatgtc accactgcc agtgaatct 
caacggagc gaaacatta ctaagggtt ggtgcagga 
ttttaccag tttttttgt ttatgacaa ttatttggc 
tgttgtgat tttttaact gcattttta acgtgcgta 
tgaatgtga agaaaaaaa acaatgctg tgtagattg 
atttaaaga aattgatgt attatta

    If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
    Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.

    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY