Status
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Gene information
General information
TC005490 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 12159376..12161319
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC005490
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes

    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_03964

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%

    embryo/egg with embryonic tissue potentially present, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
     embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC005490 RA
      MTHPCTACL SKFTTCVVI PQRYLLVIM ISLSILNAY 
TMRVCLSMT ITQMVVKKN VTESTTESV CPSDKTFKG 
FEMRKETVY DWDEKKQGL ILSSFYWGY FITHIPGGL 
LAEKLGGKH VLGIGMLTN AVLTILFPF ILKGSEGDW 
ITVVVLRVV MGLGQGALY PTVAVLLAQ WIPLGERGT 
ASSISFAGA MMGTVISNS LSGVLMTHY EGWEVVFYF 
FGVWGLVWN VFWQLVCYS YPRTNPCLK PQEKEFLDQ 
ELKEVSENK PPTPWKEIL SSLPVWALI VGQFGHDWG 
WFTMVSDLP KYMDSVLRF HVQSNGLWS AFPYLLMWG 
ATIGAGYLA DFVINREYL SVANTRRVL STVGTMGPA 
IFMVAASYV GCNKIAAVM MFTLSLVMM GFFYRGMKV 
NSIDLSPNF AGTVMALNN GVGVFAGMA APAVVGLLA 
PNQTMDEWR TVFWVSFGI FAFTNVIYV VFMSGKVQK 
WNDLKREQV EGFGK
    CDS sequence
    • >TC005490 RA
      atgacgcat ccctgtacc gcctgtttg agcaaattc 
accacctgt gtcgtaatc ccacagcgc tacttgttg 
gtaattatg atcagtctc tcaatcctc aacgcgtac 
acgatgcgt gtttgtctc tccatgacc ataacccag 
atggtagtc aagaaaaac gtcacggaa agcaccaca 
gaatcggtc tgtcccagc gataaaacc ttcaaaggc 
ttcgaaatg cgcaaagaa acggtctat gactgggac 
gagaagaag caaggtctc attttaagt tcgttttat 
tggggctac tttataacc catatcccg ggcggcctt 
ttagccgaa aaactcgga ggcaaacac gtgctagga 
atagggatg ttaaccaac gccgttctg accattttg 
ttccctttt attctaaag ggctcggaa ggcgattgg 
ataactgtg gtggtactt cgggtcgtc atgggtctg 
ggtcagggg gctttgtac ccaacggtg gccgtgctt 
ctagcccaa tggatccca cttggggaa cgtggtact 
gcctcttcc ataagtttc gctggggcg atgatgggt 
actgttatt tccaactcg ctaagtggg gtactcatg 
acccattac gagggctgg gaggtggtg ttttacttt 
ttcggggtt tggggactc gtttggaac gtgttctgg 
cagttggtt tgctactcg tacccaagg actaaccca 
tgtttgaag ccccaggag aaggagttc ctggatcag 
gagttgaag gaggtcagt gagaacaaa cctccaacc 
ccatggaag gaaattctg agttcgttg cccgtctgg 
gcgttgatt gtgggccag tttgggcac gactggggt 
tggtttacc atggttagc gatttgccc aagtacatg 
gactcggta ctgcgcttc cacgtccag agcaatggg 
ttatggtcg gcttttccc tacttgttg atgtggggg 
gcgaccatt ggtgctggg tacctagcc gactttgtt 
atcaacagg gagtacctt tccgtggcc aacaccaga 
cgagttctg tcgactgtt ggtactatg ggcccggcc 
attttcatg gttgcggcg tcctatgtc ggttgtaat 
aaaatagca gctgtgatg atgttcact ttgtcgttg 
gttatgatg ggttttttc tatcgtggg atgaaagtg 
aactcgatt gatttatcg cccaatttt gctggtact 
gtcatggca ctcaataat ggtgtgggc gttttcgcg 
ggaatggca gccccggct gtggtgggg cttctggca 
ccaaatcaa actatggac gaatggagg acggttttt 
tgggtcagt tttgggatt tttgcattc accaatgtc 
atatacgtg gtgttcatg tcggggaaa gtccagaaa 
tggaacgat ttgaagcgg gaacaagtc gagggtttt 
ggcaaataa
    mRNA sequence
    • >TC005490 RA
      gatctgacc tgacctgac ctgacctaa cagagttta 
acaaaactt gacaaaaat ttttaatgt tctcgcaaa 
gttcaagag aaaatttag aataaatag atgtctaga 
aagtagtta ttgactaga tctgacctg acatgtgat 
aattttcaa agttgcaaa cttagtatt tgttacttt 
cgtccagaa atggaacag tttgaacga acaaggaat 
aatccaact tgtagtcag tcactaaac aataaatta 
aattttcca aatgtgtca caaacattt ctgacatga 
cgcatccct gtaccgcct gtttgagca aattcacca 
cctgtgtcg taatcccac agcgctact tgttggtaa 
ttatgatca gtctctcaa tcctcaacg cgtacacga 
tgcgtgttt gtctctcca tgaccataa cccagatgg 
tagtcaaga aaaacgtca cggaaagca ccacagaat 
cggtctgtc ccagcgata aaaccttca aaggcttcg 
aaatgcgca aagaaacgg tctatgact gggacgaga 
agaagcaag gtctcattt taagttcgt tttattggg 
gctacttta taacccata tcccgggcg gccttttag 
ccgaaaaac tcggaggca aacacgtgc taggaatag 
ggatgttaa ccaacgccg ttctgacca ttttgttcc 
cttttattc taaagggct cggaaggcg attggataa 
ctgtggtgg tacttcggg tcgtcatgg gtctgggtc 
agggggctt tgtacccaa cggtggccg tgcttctag 
cccaatgga tcccacttg gggaacgtg gtactgcct 
cttccataa gtttcgctg gggcgatga tgggtactg 
ttatttcca actcgctaa gtggggtac tcatgaccc 
attacgagg gctgggagg tggtgtttt actttttcg 
gggtttggg gactcgttt ggaacgtgt tctggcagt 
tggtttgct actcgtacc caaggacta acccatgtt 
tgaagcccc aggagaagg agttcctgg atcaggagt 
tgaaggagg tcagtgaga acaaacctc caaccccat 
ggaaggaaa ttctgagtt cgttgcccg tctgggcgt 
tgattgtgg gccagtttg ggcacgact ggggttggt 
ttaccatgg ttagcgatt tgcccaagt acatggact 
cggtactgc gcttccacg tccagagca atgggttat 
ggtcggctt ttccctact tgttgatgt ggggggcga 
ccattggtg ctgggtacc tagccgact ttgttatca 
acagggagt acctttccg tggccaaca ccagacgag 
ttctgtcga ctgttggta ctatgggcc cggccattt 
tcatggttg cggcgtcct atgtcggtt gtaataaaa 
tagcagctg tgatgatgt tcactttgt cgttggtta 
tgatgggtt ttttctatc gtgggatga aagtgaact 
cgattgatt tatcgccca attttgctg gtactgtca 
tggcactca ataatggtg tgggcgttt tcgcgggaa 
tggcagccc cggctgtgg tggggcttc tggcaccaa 
atcaaacta tggacgaat ggaggacgg ttttttggg 
tcagttttg ggatttttg cattcacca atgtcatat 
acgtggtgt tcatgtcgg ggaaagtcc agaaatgga 
acgatttga agcgggaac aagtcgagg gttttggca 
aataaaaaa caattttct tggtgtgat tattttatt 
ggggaacta taaaactac tagaggggc ttgggtcaa 
cttcggtac tattccagt cctgttcat cggtcgaaa 
ctaaagcta tgaaaagta t

    If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
    Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.

    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY