Status
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Gene information
General information
TC005315 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 14530266..14533353
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC005315
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes

    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_06398

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection

    Fertility
    previtellogenic egg chamber early potentiallynot present - Number of animals showing the phenotype: 4

    previtellogenic egg chamber late potentiallynot present - Number of animals showing the phenotype: 4
    egg chamber partially not present - Number of animals showing the phenotype: 4

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    Fertility
    egg production: number no/almost no eggs -
    mature egg not present - Number of animals showing the phenotype: 4

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC005315 RA
      MSCNCATPR GIEVKNSDT QSEVSESGT ATSGFNSNW 
GSREELRDF VAKHGLKDI SDLYQDRFR VDRKKLEQM 
LSGDNDALI PADVFFANI MEDTDTYIT WPNKLKLGA 
KSKKDPHVR IAGKEEDVK AAKDRIMTV LYTRCNRVT 
MKMDISYTD HSHIIGRGG LSIKRVMEE TQCHVHFPD 
SNRSNPMEK SNQVSISGD LEGVENARS RVRELIPLI 
FGFEPPIMA QNIDNTCPY VIKIQEQYK VQVMFKTRS 
KLHATIVLV KGVEWEVEQ VKQATILLM EYMCENLTN 
QIPVQMTIQ ISPQHHAIV LGKNHSNLK AIMHYTKCQ 
IMFPDAQDQ NIPSLKRSN VNISGNIHS VYTARQLLI 
GSLPLLIIF DLPEDIANL KVRSEQIDE IQNTCDVTI 
TVRQKSKQM IKACIIKGI ERQASNIYK ARNLILGID 
DPPIQADIP RLYHIPSTP SPNNDLLTT PTNLNPSPL 
TPIFTWHFS SDQTTPFEI VQKPQMLYN NNNNKMQQS 
SNSSGFQSF EQVKADTSL LSSISSNSS NYVSSPPTV 
FSPQSDNNI PTTSDYQSM NEKKSASFD YDCSKRLAG 
FKAMQGRPN PDVYRVPTA MWAGYGISH TSPAGMLKK 
EDDIWKPAE VFPSTSGLH NTSNILDHT PTHFINRIA 
NTTWSDLPS LLSALGLDR YISLFIRHE VDLPTFTTL 
SDKDLMTIG VTAFGSRRK MLLAISELN KRSCTFSAA 
PGAERKSSS SNSPPSHDN SPRQSW
    CDS sequence
    • >TC005315 RA
      atgtcttgt aattgtgct acacctcgt ggaatcgag 
gtaaaaaat tccgacaca caatcagaa gtgtcggaa 
agcggcacg gctacttct ggtttcaac tcaaattgg 
ggctccaga gaggaatta agggatttt gtcgccaaa 
cacggatta aaggacatt tcggatttg taccaggac 
cggttccgg gtcgaccgc aaaaagttg gaacagatg 
cttagtggg gacaacgat gcactgatc cctgctgac 
gttttcttt gcgaacatt atggaagac actgacact 
tacataacg tggccaaac aaactcaaa ttgggggct 
aaatcgaaa aaggatcct cacgtgagg atcgccggc 
aaagaagag gatgttaaa gccgccaaa gacaggatt 
atgacggtt ttgtacacg agatgcaat cgtgtaacc 
atgaaaatg gacataagt tacacggac cattcgcat 
attatcggc agaggaggg ctgagtatt aaacgtgtc 
atggaagaa actcaatgc cacgtacat ttccctgat 
tcgaatcgt tcgaatccg atggaaaaa agcaatcaa 
gtttcgatt tcgggcgat ttggaaggg gttgaaaat 
gcaaggagt cgcgttagg gaattaatt ccgctgatt 
tttgggttt gagcctcca ataatggcc caaaatatt 
gataatacg tgtccttac gtaattaaa atccaggaa 
cagtacaaa gtgcaagtc atgttcaaa actaggtcc 
aaacttcat gctactata gtactagtc aagggagtc 
gaatgggag gtggagcag gtgaagcag gctacaatt 
ttgctcatg gaatacatg tgcgaaaat ttgacgaac 
caaattccg gttcaaatg actatccaa atttcgccc 
caacaccat gcaatcgtt ttgggcaaa aatcattca 
aacctaaag gcaatcatg cactacacc aaatgccaa 
atcatgttt cctgatgcc caggaccaa aatatcccg 
agtttgaag cgaagtaat gttaatatt tctggaaat 
atacatagt gtttatacc gctcgtcaa ctgctaatt 
ggaagtttg ccactcttg attattttc gacttgcct 
gaagatatt gcaaatctc aaagttcgt tctgaacaa 
atcgacgaa atccaaaat acttgtgac gtcacaatt 
actgtaaga caaaagtca aagcagatg attaaagct 
tgcatcatc aagggcatc gaacggcaa gcttcaaac 
atatacaag gcccgaaat ttaatcctg ggtatcgac 
gatccgccc atccaggcc gatattccc cgcctttac 
cacatccca agcactccg tcgcccaat aatgacctg 
ttgaccact cccactaac ctaaacccc tcccctttg 
accccgata ttcacttgg catttttcc tctgaccaa 
accacgccc tttgaaatt gtccaaaag ccacagatg 
ttgtacaac aacaacaac aacaagatg caacaatcg 
tccaacagt agcggtttc caatcgttt gaacaagtc 
aaagctgat actagcctc ctgtctagt atttccagc 
aatagtagt aactacgtg tcaagccct ccaacggtg 
ttttcgccc caaagtgat aataacatc cccactact 
tctgattac caatcaatg aatgagaaa aagtccgcc 
agtttcgat tatgactgc agcaagcgt ttggctggt 
ttcaaggcg atgcagggg cgtccaaac cctgatgtg 
tatagggtg cccaccgct atgtgggca gggtacggc 
ataagtcac acaagtcct gctggaatg ttgaaaaaa 
gaagacgat atctggaaa cccgcagaa gtgtttcca 
tcaacatca gggcttcac aacactagt aacattcta 
gaccacact cccacccac tttataaac cgcattgcc 
aacaccact tggtctgat ttgccgtca cttctaagt 
gccttaggt ttggaccgt tatatcagt cttttcatt 
cgacatgaa gttgatttg ccaacgttt acgactctt 
tccgacaaa gatttgatg acaatcgga gtgacagca 
ttcggaagt cgtcgcaaa atgctattg gctatctcg 
gaactcaac aagagaagt tgtacgttt tcggcggct 
cctggagca gagcgcaaa agttcctct tcgaattcg 
cccccaagt catgacaac agtcctcgt caaagttgg 
taa
    mRNA sequence
    • >TC005315 RA
      agtcgcgca aattaccaa aagcgtgtc acctacaca 
gcccttaaa aacgcgtaa aaatcctta caaagtgac 
cattaaatg gggttagtc attaattaa ttgtggacg 
tagtgattt aaaaaaaaa tgtcttgta attgtgcta 
cacctcgtg gaatcgagg taaaaaatt ccgacacac 
aatcagaag tgtcggaaa gcggcacgg ctacttctg 
gtttcaact caaattggg gctccagag aggaattaa 
gggattttg tcgccaaac acggattaa aggacattt 
cggatttgt accaggacc ggttccggg tcgaccgca 
aaaagttgg aacagatgc ttagtgggg acaacgatg 
cactgatcc ctgctgacg ttttctttg cgaacatta 
tggaagaca ctgacactt acataacgt ggccaaaca 
aactcaaat tgggggcta aatcgaaaa aggatcctc 
acgtgagga tcgccggca aagaagagg atgttaaag 
ccgccaaag acaggatta tgacggttt tgtacacga 
gatgcaatc gtgtaacca tgaaaatgg acataagtt 
acacggacc attcgcata ttatcggca gaggagggc 
tgagtatta aacgtgtca tggaagaaa ctcaatgcc 
acgtacatt tccctgatt cgaatcgtt cgaatccga 
tggaaaaaa gcaatcaag tttcgattt cgggcgatt 
tggaagggg ttgaaaatg caaggagtc gcgttaggg 
aattaattc cgctgattt ttgggtttg agcctccaa 
taatggccc aaaatattg ataatacgt gtccttacg 
taattaaaa tccaggaac agtacaaag tgcaagtca 
tgttcaaaa ctaggtcca aacttcatg ctactatag 
tactagtca agggagtcg aatgggagg tggagcagg 
tgaagcagg ctacaattt tgctcatgg aatacatgt 
gcgaaaatt tgacgaacc aaattccgg ttcaaatga 
ctatccaaa tttcgcccc aacaccatg caatcgttt 
tgggcaaaa atcattcaa acctaaagg caatcatgc 
actacacca aatgccaaa tcatgtttc ctgatgccc 
aggaccaaa atatcccga gtttgaagc gaagtaatg 
ttaatattt ctggaaata tacatagtg tttataccg 
ctcgtcaac tgctaattg gaagtttgc cactcttga 
ttattttcg acttgcctg aagatattg caaatctca 
aagttcgtt ctgaacaaa tcgacgaaa tccaaaata 
cttgtgacg tcacaatta ctgtaagac aaaagtcaa 
agcagatga ttaaagctt gcatcatca agggcatcg 
aacggcaag cttcaaaca tatacaagg cccgaaatt 
taatcctgg gtatcgacg atccgccca tccaggccg 
atattcccc gcctttacc acatcccaa gcactccgt 
cgcccaata atgacctgt tgaccactc ccactaacc 
taaacccct cccctttga ccccgatat tcacttggc 
atttttcct ctgaccaaa ccacgccct ttgaaattg 
tccaaaagc cacagatgt tgtacaaca acaacaaca 
acaagatgc aacaatcgt ccaacagta gcggtttcc 
aatcgtttg aacaagtca aagctgata ctagcctcc 
tgtctagta tttccagca atagtagta actacgtgt 
caagccctc caacggtgt tttcgcccc aaagtgata 
ataacatcc ccactactt ctgattacc aatcaatga 
atgagaaaa agtccgcca gtttcgatt atgactgca 
gcaagcgtt tggctggtt tcaaggcga tgcaggggc 
gtccaaacc ctgatgtgt atagggtgc ccaccgcta 
tgtgggcag ggtacggca taagtcaca caagtcctg 
ctggaatgt tgaaaaaag aagacgata tctggaaac 
ccgcagaag tgtttccat caacatcag ggcttcaca 
acactagta acattctag accacactc ccacccact 
ttataaacc gcattgcca acaccactt ggtctgatt 
tgccgtcac ttctaagtg ccttaggtt tggaccgtt 
atatcagtc ttttcattc gacatgaag ttgatttgc 
caacgttta cgactcttt ccgacaaag atttgatga 
caatcggag tgacagcat tcggaagtc gtcgcaaaa 
tgctattgg ctatctcgg aactcaaca agagaagtt 
gtacgtttt cggcggctc ctggagcag agcgcaaaa 
gttcctctt cgaattcgc ccccaagtc atgacaaca 
gtcctcgtc aaagttggt aattagaca gttgtacca 
atcattgtg acttttgtt tgctatttg tattttgtt 
ttaattgtt gtgttgttg gagattttg gaaaattgg 
ttgttggtg attgataaa attccgcaa attatttct 
taaaaattc tgcgcgcat ttttatagc aataatctg 
gagggagtt ttatgtgta ctcgtttta taattaagc 
cgatcaatt gtgatattt tatgagcat tgccccatt 
ttcatatca tgttcatat agtttttaa ctggagtaa 
ttttaagga cagattttg taagcaaaa gttttaatt 
tgcttcttt tgattattt gattcactt tttgtacct 
tttatgaga taaattaaa acttttgta ccctgtgat 
gtaaatcct tattctact acatataat ttattgtta 
ttattataa aaactacca ttattatta ttattatta 
ttgatgctt aaagcaatt attacaaat gaac

    If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
    Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.

    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY