| General information | |||
| TC002851 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007418.3: 8146821..8153068 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC002851 | |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
| Gene | {{ goGene }} |
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| GO ID | * {{ blurIdResult }} |
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| Evidence | {{ blurEvidenceResult }} |
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| Reference | * {{ blurReferenceResult }} |
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| Quotation | ||
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| Publish name | ||
| {{ unavailableText }} |
* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_00455 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
||
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
adult nutritional state bad (cachectic) - Number of animals showing the phenotype: 4 (11 dpi)
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)
pupal molt not fulfilled (died as pupa) - Number of animals showing the phenotype: 2 (11 dpi)
pupal molt delayed (still larva) - Number of animals showing the phenotype: 3 (11 dpi)
pupal molt not fulfilled (died as L7 or prepupa) - Number of animals showing the phenotype: 3 (19 dpi)
eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 2 (19 dpi)
- >TC002851 RA
MTVTESSEH DGEKDENSA EAPSEDDKN VLTSYIQEV EERFVCKQE LRSANQNAA NTRPSESHF SKLDSSLKK NTAFVKKIK NFSANQVDT YLKDMTGLN LSKYISEIA AAIVDAKLK MTDVGAAVK MCSVLHQTY ADFSQHLFE NWQKTLAFK VGEKIPNPS KLRVDLRFY AELVQAGVF SNKNAFTLL GTVITTLVN MDKDEHYNC SIILSFCKH CGEDYAGLV PRKMRELSE KFDIPIPKS TFLPPEKQQ NVRTLLRDY YVSLSKHLV KDHQEIQNF EKQNMRILQ TKGELSQER KDKLEALQT SYDKLLAST QSFAEILDE DMPTLKNQT LMKNDESMI VTGAGPDLD EAALANIEN VWCDIETQR FYCELPELQ AFLPTSYIN KQAPPPEES VTEEVLDSE IPTEELEDD EKAEEAPVA AEDEPEDSA TTNASNKIL LEAFFNNLP NCVNREMID NAAIDFLVT LNNKHNRKK LVRALFGVN RTRLDLLPF YARFVAILR PALPEVGNE LCQMLRQDF KYHVRKKDQ INIESKIKV VRFIGELVK FKLYSKIEA LYCLKVLLY DFSHHHIEM ACNLLEVCG RFLYCSPDS HQRTRVYLE QMMRKKAVM ALDSRYVTQ IENAYYYVN PPEVTITKK ERPIMHQFI RKLLFQDLQ KNNTDKIMR LIRKLDWGN EDVSAYAIK CLSSAHNVK YFNIRCLAN LLAGLVTYQ EEVGTRVVD SVLEDIRLG MEVNLPKYN QRRVAQVKY LGELYNYRM VESADIFKV LYSLITFGV SMDPNIPSP LDPPNHLFR IRLACVLLE TCGTYFSSG SSKRRLDYY LIFLQSYYW FKRDLWKDA FPPTLEHIF KDTLTTLRP KIKLCQSYE ETQTELNNI RVTLGFDKI TGDLNENKV EEDGLETIA ETDTEEQDE VSEVTAPIT DDTATEDET VDHTQGSDD EGEPDVSEA EQEQVSESL AIPSGPRKV ECPEDEDFL NALDKMVSE NIQERMREP VKAGNVDIS VPVVLKSNK KTYEQLQDA PETEPNKIG FVLMVRKGN KQQYKNFEA DINSELAQN LRDQELAQK EEKERVKRL TLNITERFE EEDYQDMMQ QQNRPVTQN LNRERKKYQ HPKGAPDAD LIFGPKRVR
- >TC002851 RA
atgaccgtg accgaatcc tcggaacac gacggtgag aaagacgag aacagtgcc gaggccccc agcgaagac gacaaaaac gtcctaacc tcttacatc caagaggtg gaagagagg tttgtttgc aagcaagag ctgcgaagt gccaaccaa aatgcggca aatacgcgc cccagcgag tcccacttc agcaagctg gactccagc ctcaagaag aacacggcg tttgttaag aaaatcaaa aacttctct gccaaccaa gtggacaca tacttgaaa gacatgacg gggctgaac ctgtccaag tacatatcg gagattgcg gctgcgata gtcgatgct aaattgaaa atgaccgat gtgggcgca gccgtaaag atgtgcagt gttttgcac cagacttac gctgatttc tcgcaacac ttgtttgag aactggcag aaaaccctg gcctttaaa gtgggtgag aagattcca aatccgagt aaactgagg gtggattta aggttttat gccgagttg gtgcaggct ggggtgttt agcaataaa aacgcgttc actttgctg gggaccgtg atcaccact ttggtcaac atggacaag gacgaacac tacaattgc tcaataatc ctcagcttt tgcaagcac tgcggcgaa gattacgca ggtctggtc cctcgcaag atgcgggaa ttgtccgaa aaattcgac atccccatc ccgaagagc actttcctg ccgcccgag aagcagcaa aacgtgcgc accttgctg agggattac tacgtttcg ctcagtaag catctcgtc aaagaccac caagagatc caaaacttc gagaagcag aacatgagg atactccaa accaaaggc gagttgagt caagaacga aaagacaag ctcgaggcg cttcaaaca tcgtacgat aaattatta gcttcgaca caaagtttt gcggaaatt ttagacgaa gatatgcca actttgaaa aatcagact ctcatgaaa aatgatgag agtatgata gtgacaggt gcggggccc gatttggac gaagctgcg ttggctaat atcgagaat gtttggtgc gatattgaa acgcagagg ttttactgc gagttgccg gaattgcaa gcttttttg ccgacttct tatataaat aaacaggcg cctccgccc gaggagtcg gtcactgag gaggtgctg gattcggaa ataccaact gaagaactg gaagatgac gaaaaagcc gaggaggcg cccgtggca gcggaagat gagcccgaa gactctgcc acaaccaac gccagcaat aaaatcctc ctcgaggca tttttcaac aacttgccc aactgcgtg aaccgcgaa atgatcgac aacgcagcc atcgatttc ctcgtaacc ctcaacaac aaacacaac cgcaagaag ctggtccgt gccctgttc ggggtaaac cgcacccgt ctcgacctc ctcccgttc tacgcccgc ttcgtggcc atcctccgt ccagccctt cccgaagtg ggcaacgaa ctgtgccaa atgctgcgg caagacttc aaataccac gtacgcaag aaagaccaa attaacatc gaatccaaa ataaaagtc gtacgattt atcggcgaa ctcgtcaaa ttcaagctc tattcaaaa atagaagct ctctattgt ctcaaagtt ctgctgtac gatttctcg caccatcac atcgaaatg gcctgcaat ctgttggaa gtttgcggc cggtttttg tactgcagt cccgactcg catcaaagg acgagggtg tatttggag caaatgatg aggaaaaag gccgttatg gcgttggat tcgagatat gtgacgcaa attgaaaac gcctattat tacgtcaat ccgcccgaa gtaacaatc acgaagaag gagaggccg ataatgcac cagtttatc cggaaattg ctctttcaa gacttgcag aagaataac actgataag attatgagg ctgattagg aagctggac tggggcaac gaggacgtg tccgcttat gcaataaag tgtctaagc agcgcccac aacgtgaag tatttcaat atcaggtgt ctcgcaaat ttgcttgcg ggcttggtg acctaccag gaagaggtt ggcactcgt gtagtggac agtgtttta gaagacatt cggttaggt atggaagta aatcttccc aaatataac cagagacgt gtagctcaa gtaaaatat cttggggag ctgtataac tacagaatg gttgaaagc gctgacatt ttcaaggtt ttgtattct ttgataaca tttggagtt tcaatggat ccaaacata ccgtcgcca ctcgatcca ccaaatcac cttttccga ataagactg gcttgtgtt ttacttgaa acatgcggc acgtatttc agcagcggt agcagtaag aggagacta gactattat ttaatattt ctgcaatcg tattattgg ttcaagcgc gacttgtgg aaagatgca tttcctcca acactggag cacattttt aaagacact ctcacgacc ctgagacca aaaattaaa ttgtgtcaa agctacgaa gaaacacaa accgagtta aataatatt cgcgtcact ttgggcttt gataaaatc actggagat ttgaacgaa aataaagtt gaggaagac ggcctggaa acgatcgct gaaaccgac actgaagaa caggacgaa gtgtccgaa gtcacggca cctattact gatgatacc gccactgag gacgaaacg gtggaccac acacaaggc tcggacgat gaaggggaa cccgatgtt agcgaagct gagcaagaa caagtgtcc gaatcgctg gcaattccc agtggcccc cgcaaggtc gagtgcccc gaggacgag gacttttta aacgctttg gataaaatg gtttcggag aacatccaa gagcggatg cgcgagccg gtgaaggcc ggaaacgtg gacatttcc gttccggtt gtcctcaaa tcgaacaaa aagacctac gagcagttg caggacgcc cctgaaacg gaacccaac aaaatcggg ttcgtgcta atggtccgc aaaggaaac aagcagcaa tataaaaat ttcgaggct gatatcaac tccgaactc gctcagaat ctgagggac caggagttg gctcagaag gaggagaag gaacgagtg aagcgcttg acgctgaac atcacggaa cggtttgaa gaggaggat tatcaagac atgatgcag cagcagaac aggcccgtg acccaaaac ttgaaccgc gaacgcaag aaataccag caccccaag ggggcgccc gacgccgac ctgattttt ggacctaag agagttaga taa
- >TC002851 RA
tgtacattt gtccgggtt aaatccgaa ttattgtct ttaattttg atgatgtaa acactttcc gttcgcttg gaattaacg ataagataa cacgaaagt tgattgtgt aacttgcat tggcaacac ttatatcta ttgacggtt gggaatttt cgctttttc cctaaattg ttttctaat aataatcat tttaacaag ttttttgca aataaagcc cgttttgcg acatgaccg tgaccgaat cctcggaac acgacggtg agaaagacg agaacagtg ccgaggccc ccagcgaag acgacaaaa acgtcctaa cctcttaca tccaagagg tggaagaga ggtttgttt gcaagcaag agctgcgaa gtgccaacc aaaatgcgg caaatacgc gccccagcg agtcccact tcagcaagc tggactcca gcctcaaga agaacacgg cgtttgtta agaaaatca aaaacttct ctgccaacc aagtggaca catacttga aagacatga cggggctga acctgtcca agtacatat cggagattg cggctgcga tagtcgatg ctaaattga aaatgaccg atgtgggcg cagccgtaa agatgtgca gtgttttgc accagactt acgctgatt tctcgcaac acttgtttg agaactggc agaaaaccc tggccttta aagtgggtg agaagattc caaatccga gtaaactga gggtggatt taaggtttt atgccgagt tggtgcagg ctggggtgt ttagcaata aaaacgcgt tcactttgc tggggaccg tgatcacca ctttggtca acatggaca aggacgaac actacaatt gctcaataa tcctcagct tttgcaagc actgcggcg aagattacg caggtctgg tccctcgca agatgcggg aattgtccg aaaaattcg acatcccca tcccgaaga gcactttcc tgccgcccg agaagcagc aaaacgtgc gcaccttgc tgagggatt actacgttt cgctcagta agcatctcg tcaaagacc accaagaga tccaaaact tcgagaagc agaacatga ggatactcc aaaccaaag gcgagttga gtcaagaac gaaaagaca agctcgagg cgcttcaaa catcgtacg ataaattat tagcttcga cacaaagtt ttgcggaaa ttttagacg aagatatgc caactttga aaaatcaga ctctcatga aaaatgatg agagtatga tagtgacag gtgcggggc ccgatttgg acgaagctg cgttggcta atatcgaga atgtttggt gcgatattg aaacgcaga ggttttact gcgagttgc cggaattgc aagcttttt tgccgactt cttatataa ataaacagg cgcctccgc ccgaggagt cggtcactg aggaggtgc tggattcgg aaataccaa ctgaagaac tggaagatg acgaaaaag ccgaggagg cgcccgtgg cagcggaag atgagcccg aagactctg ccacaacca acgccagca ataaaatcc tcctcgagg catttttca acaacttgc ccaactgcg tgaaccgcg aaatgatcg acaacgcag ccatcgatt tcctcgtaa ccctcaaca acaaacaca accgcaaga agctggtcc gtgccctgt tcggggtaa accgcaccc gtctcgacc tcctcccgt tctacgccc gcttcgtgg ccatcctcc gtccagccc ttcccgaag tgggcaacg aactgtgcc aaatgctgc ggcaagact tcaaatacc acgtacgca agaaagacc aaattaaca tcgaatcca aaataaaag tcgtacgat ttatcggcg aactcgtca aattcaagc tctattcaa aaatagaag ctctctatt gtctcaaag ttctgctgt acgatttct cgcaccatc acatcgaaa tggcctgca atctgttgg aagtttgcg gccggtttt tgtactgca gtcccgact cgcatcaaa ggacgaggg tgtatttgg agcaaatga tgaggaaaa aggccgtta tggcgttgg attcgagat atgtgacgc aaattgaaa acgcctatt attacgtca atccgcccg aagtaacaa tcacgaaga aggagaggc cgataatgc accagttta tccggaaat tgctctttc aagacttgc agaagaata acactgata agattatga ggctgatta ggaagctgg actggggca acgaggacg tgtccgctt atgcaataa agtgtctaa gcagcgccc acaacgtga agtatttca atatcaggt gtctcgcaa atttgcttg cgggcttgg tgacctacc aggaagagg ttggcactc gtgtagtgg acagtgttt tagaagaca ttcggttag gtatggaag taaatcttc ccaaatata accagagac gtgtagctc aagtaaaat atcttgggg agctgtata actacagaa tggttgaaa gcgctgaca ttttcaagg ttttgtatt ctttgataa catttggag tttcaatgg atccaaaca taccgtcgc cactcgatc caccaaatc accttttcc gaataagac tggcttgtg ttttacttg aaacatgcg gcacgtatt tcagcagcg gtagcagta agaggagac tagactatt atttaatat ttctgcaat cgtattatt ggttcaagc gcgacttgt ggaaagatg catttcctc caacactgg agcacattt ttaaagaca ctctcacga ccctgagac caaaaatta aattgtgtc aaagctacg aagaaacac aaaccgagt taaataata ttcgcgtca ctttgggct ttgataaaa tcactggag atttgaacg aaaataaag ttgaggaag acggcctgg aaacgatcg ctgaaaccg acactgaag aacaggacg aagtgtccg aagtcacgg cacctatta ctgatgata ccgccactg aggacgaaa cggtggacc acacacaag gctcggacg atgaagggg aacccgatg ttagcgaag ctgagcaag aacaagtgt ccgaatcgc tggcaattc ccagtggcc cccgcaagg tcgagtgcc ccgaggacg aggactttt taaacgctt tggataaaa tggtttcgg agaacatcc aagagcgga tgcgcgagc cggtgaagg ccggaaacg tggacattt ccgttccgg ttgtcctca aatcgaaca aaaagacct acgagcagt tgcaggacg cccctgaaa cggaaccca acaaaatcg ggttcgtgc taatggtcc gcaaaggaa acaagcagc aatataaaa atttcgagg ctgatatca actccgaac tcgctcaga atctgaggg accaggagt tggctcaga aggaggaga aggaacgag tgaagcgct tgacgctga acatcacgg aacggtttg aagaggagg attatcaag acatgatgc agcagcaga acaggcccg tgacccaaa acttgaacc gcgaacgca agaaatacc agcacccca agggggcgc ccgacgccg acctgattt ttggaccta agagagtta gataatggg aggaattca ggatgaaat ggttgaata cgattaagg gtcaaagaa gttcagaat gtttcagaa atagataga ttgtttcgt ttttagtct atcggaatt gagaattgt gaaggagaa gcaaggaat ttcattttt tgtttcgtt caac
If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.
