Status
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Gene information
General information
TC002616 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 21452451..21467469
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC002616
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes

    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_00414

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection

    remarks: animals lost,
    Pupal morphology
    remarks: animals lost,
    Adult morphology
    remarks: animals lost, prothoracic odoriferous glands partially colour darker - Number of animals showing the phenotype: 8 (41 dpi)
    Days post injection


    Fertility
    remarks: animals lost,
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC002616 RA
      MSGHLDNPA FINDDGKSN HSFTPALNS TEDLNRKNE 
KNYVVEMKD LKKAEEATG EYEPYLHRD VEHPTTNAE 
TLLHLLKGS LGTGILSMP LAFFHSGYL VGIICTILI 
GGICTYCIH LIIQAEYEL CKRKKMPSL TYPATAELA 
LLEGPKFFQ VLAPYSVHV INTFLLIYQ LGACCVYTV 
FIAENVKHV ADEYIEKLD VKIWMLVIL LPLILINYI 
RNLKFLAPF STVANFITI VSFGIILYY LIKADMTFE 
GRNVAGKIA DFPLYFGTV LFALEAIGV IMPLENEMK 
TPKAFKGGC GVLNIGMIS IVVLYVGMG LLGYIAYGS 
DVADTITIN LSPEDVLAQ VAKIMLAIA IYITHPLQM 
YVAIDIIWN EYLASRFEK SRYQLFFEY AVRTALVLI 
TFALAVAIP KLDLFISLF GAFCLSALG LAFPAIIQT 
STFWYSLTG FSGKMVIAK NCALVLFGI IGLIVGTYT 
SLQKIVEFF NE
    CDS sequence
    • >TC002616 RA
      atgagtgga caccttgat aacccggct tttatcaac 
gacgacggc aagagcaac cacagcttt actccggcg 
ctcaattct acggaagac ctgaaccgg aagaatgaa 
aaaaactac gtcgttgag atgaaggat ttgaagaaa 
gctgaggaa gccaccggc gaatatgag ccctatttg 
caccgagac gtcgaacat ccgaccaca aatgccgaa 
acattgctg catctcctt aagggcagc ttgggcact 
ggaatcctc tcaatgcct ttggccttc ttccattcc 
ggatactta gtagggatc atttgcaca attctaata 
ggcgggatt tgcacgtac tgcatacat ttgatcatc 
caagcggaa tatgagctg tgcaaacgg aaaaaaatg 
cccagttta acgtaccca gcaacggcc gaactggcc 
ctgcttgaa ggcccaaaa ttcttccaa gttctagct 
ccctactct gtacatgtt ataaatacg ttcttactg 
atatatcaa ctgggggct tgttgtgtg tacacagtg 
tttatcgcc gaaaatgtg aaacatgtc gctgatgaa 
tatatcgaa aaattggac gtgaaaatc tggatgttg 
gtcatcctc ctacctcta atactcatc aattacatc 
agaaatttg aaatttctg gcccccttt tccacagtt 
gccaacttt attaccatt gttagtttt ggaattatt 
ttgtactac ctcattaaa gccgacatg acatttgaa 
ggacgaaac gttgcagga aaaattgcc gattttccc 
ctttatttt ggaaccgtg ctgtttgct ctagaagcc 
atcggtgtg ataatgccg ctcgaaaac gaaatgaaa 
actcccaaa gctttcaaa ggcggctgt ggagtccta 
aatattggc atgatttcc attgtggtt ttatacgtc 
gggatgggt ttgctgggc tatatcgcc tatgggtct 
gatgttgca gacaccatc accattaac ttatcccca 
gaggatgtc ctagcacag gtggctaaa ataatgtta 
gccatagct atctacatc acgcatcct ctgcagatg 
tacgtggct attgatatt atatggaac gaatattta 
gcgtcaaga tttgaaaaa agccgctac cagctgttt 
ttcgaatat gctgtgcga actgctcta gtcttaatt 
acatttgcc ttagcggtg gccattccg aagttggat 
ctctttata tcattgttt ggtgctttc tgtctgtca 
gcgttaggt ttagccttc cctgcgatc atccagacg 
agtactttt tggtactcg ctgactggg ttcagtggc 
aaaatggtg attgccaaa aactgtgca cttgtccta 
tttggcatt ataggactg atcgtagga acgtacaca 
agtttacaa aaaattgtc gagtttttc aatgaataa
    mRNA sequence
    • >TC002616 RA
      tgtttattg tttgcaacg ctgatgtta acaactaca 
gtggcggct tttattgtt atgtgttta gtttcgaaa 
actggaggg actgcaatt caggcgaaa atagccgaa 
tttaattaa gatcaaatt ttccatcac caacaacct 
gaacaatga gtggacacc ttgataacc cggctttta 
tcaacgacg acggcaaga gcaaccaca gctttactc 
cggcgctca attctacgg aagacctga accggaaga 
atgaaaaaa actacgtcg ttgagatga aggatttga 
agaaagctg aggaagcca ccggcgaat atgagccct 
atttgcacc gagacgtcg aacatccga ccacaaatg 
ccgaaacat tgctgcatc tccttaagg gcagcttgg 
gcactggaa tcctctcaa tgcctttgg ccttcttcc 
attccggat acttagtag ggatcattt gcacaattc 
taataggcg ggatttgca cgtactgca tacatttga 
tcatccaag cggaatatg agctgtgca aacggaaaa 
aaatgccca gtttaacgt acccagcaa cggccgaac 
tggccctgc ttgaaggcc caaaattct tccaagttc 
tagctccct actctgtac atgttataa atacgttct 
tactgatat atcaactgg gggcttgtt gtgtgtaca 
cagtgttta tcgccgaaa atgtgaaac atgtcgctg 
atgaatata tcgaaaaat tggacgtga aaatctgga 
tgttggtca tcctcctac ctctaatac tcatcaatt 
acatcagaa atttgaaat ttctggccc ccttttcca 
cagttgcca actttatta ccattgtta gttttggaa 
ttattttgt actacctca ttaaagccg acatgacat 
ttgaaggac gaaacgttg caggaaaaa ttgccgatt 
ttccccttt attttggaa ccgtgctgt ttgctctag 
aagccatcg gtgtgataa tgccgctcg aaaacgaaa 
tgaaaactc ccaaagctt tcaaaggcg gctgtggag 
tcctaaata ttggcatga tttccattg tggttttat 
acgtcggga tgggtttgc tgggctata tcgcctatg 
ggtctgatg ttgcagaca ccatcacca ttaacttat 
ccccagagg atgtcctag cacaggtgg ctaaaataa 
tgttagcca tagctatct acatcacgc atcctctgc 
agatgtacg tggctattg atattatat ggaacgaat 
atttagcgt caagatttg aaaaaagcc gctaccagc 
tgtttttcg aatatgctg tgcgaactg ctctagtct 
taattacat ttgccttag cggtggcca ttccgaagt 
tggatctct ttatatcat tgtttggtg ctttctgtc 
tgtcagcgt taggtttag ccttccctg cgatcatcc 
agacgagta ctttttggt actcgctga ctgggttca 
gtggcaaaa tggtgattg ccaaaaact gtgcacttg 
tcctatttg gcattatag gactgatcg taggaacgt 
acacaagtt tacaaaaaa ttgtcgagt ttttcaatg 
aataagttt gccataata tgattgtgt ttagcggaa 
actcatgat tctgaagta caagaaaga agtaagaat 
tttgtaatg tgatataag ttcaatgct caatgtcct 
agtacatta ggcacaatg ttatatagt aaattatta 
ttttatatt gtgttatat tacgtgtat atacagatt 
aaggctgtt cgactttgt aaagtgaat tgagggttc 
attgtaagg actctacct attttctta cacgagtag 
tgtatattg tagctgcct cttttacta ttacaacta 
tttttattt tctcatttt gccataatg agacataca 
actaaataa agatgctta agtaac

    If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
    Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.

    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY