| General information | |||
| TC002437 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007418.3: 29121986..29128288 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC002437 | |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
| Gene | {{ goGene }} |
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| GO ID | * {{ blurIdResult }} |
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| Evidence | {{ blurEvidenceResult }} |
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| Reference | * {{ blurReferenceResult }} |
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| Quotation | ||
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| Publish name | ||
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_08055 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)
- >TC002437 RA
MGNTHAKPH PKPRNKKQV HWKAADRPS PPGKPHLSD NDLTPDLVT IVWSKPTRD GGSPITGYL VEHRRTGSP HWVRATPLL VPFPELTLS GLEPGWRYQ FRVRAENAV GLSEPSDIS EPLTVTLQK FAITAPKFT EELKDATAL ENEKVEFVV HFLGQPAPK ICWFKDGFE IFSSRRTRI LTENDRSVL TIHQSALSD EGEIKCTAT NKAGHASTK ARLSLEAPP SIRLPRQYE DGLLFEIGE VIRLKVSVA GRPTPLVFW SHDGESIQN NDRYEIEYV DKCSILKIA EATRGDRGE YQIKAVNKI GEDLASFLV TITDKPSPP GRARVVMTL GRSVTLSWS TPDDDGGCK IGNYIVEYY RLGWNVWLK AATSRQLTT ILGDLIEGS EYKFRIKAE SPYGISEPG AESDIVFIP DPKRGITSP QARSRSQPK DIIDEITSV PVAAKRRPK PRSQSSITK AETNYYDEM PKRPERNKI KSPPKTPEV VRKNASPQT ILDRASLAR ELAYGSPEI KMLQSERPT SPLPPQTKS PSPPKSRTP SPNLTPSPS EDKSKTPSP KDKRSLLRE KSETFEESS EFMLVLYPD ANEKGRRRS SQNIEFDFD EYDIPPPLS LSAPELGAE PPVFENLKT SASSTELLH ERAMMRFNE AAAAEEEEL KKRRKFSYD GQHNIDIPK IQINSKDDQ DIVGLERKP SRRKSSGSV VQQQLLWAQ KRFSLKNSN DLNEFIETK LQKTQIPSP EAPKKEMVR QRSESEERE EEEFNRVRA KMGLQGQSS LEKRSIEVV DEEKWLEDY EESLSESET ESEDERDYD IQPPQRIYS DEDEETYHP GVMTTPRKP SVSDEPFEI LTKRNKLPD PNFVPKPIL KKTEVEPPK VSSTKIRSH SPMPQFRQE VTRGRLQSL AQPPIELSD NKSKVFQRS ISLSTEENT ESEKIAPTL QAAPGRNIS AVATLCGIT AASIVIPEK LLQKKNDEE ESKVVVDHY MDIVRSYGQ RKKSNPEVW KAATNEAKT WSTTKEEKT EEEAQTLMS YGQRKNSNP QIRKVLEET KPEAKTWST NKEEKDEEL TEIVKSSGQ RKNSVPQSL KEEVKTWNT TKEEKSEVQ PLRTERSPS PSPQKTAPN QKRGRSGVN HKPRRGSSR SKTPSRSPS RLENWSGRK SSPSPMKRK TSPSPMRAR KSPSPIPLA KPRPKLREI TTQTSIGLE LNYSSDSRA STPHDRRQE ELLAKAEVK VRSFVDYLT DLAMFFVAC WLYLFSNEL LAIPVLLVM VYRQLKNEI GKRIPRWIV RRFSKKKAK K
- >TC002437 RA
atggggaac acacacgcg aagccccat ccaaaaccg aggaataaa aaacaagta cactggaag gctgcagac cgcccctcc ccgcccggc aagccccac ctctccgac aacgacctc acccccgac ctcgtcacc atcgtctgg tctaagcca acccgcgat ggcggctct ccaatcacc ggctacctc gtcgagcac cgccgcaca ggctccccc cactgggtg cgagccacg cccctcctc gtccccttc cccgagctc accctcagc ggcctggag cccggctgg cgctaccag ttccgggtg cgggccgaa aacgcagtt ggcctttcc gaacccagc gacatctcc gaaccctta actgtaacg ttgcaaaaa ttcgctatt acagcacca aagttcacc gaagaactc aaagacgca acagcgctc gaaaatgag aaagttgag ttcgttgtc cactttttg ggacaacct gcgccgaaa atctgctgg tttaaggac gggtttgag atttttagc agcagaagg acgaggatt ttgacggag aatgatcgg agtgtgttg actatacac cagagcgcg ctgtcggat gagggcgag atcaagtgc acagcgacc aataaggcg gggcatgcg tccacgaag gcgcggcta agcttggaa gccccgccc agtatacgc ctaccgaga caatacgaa gatgggctg ttgtttgag atcggggaa gttattagg ttgaaggtt tctgtggct ggccgtccg actccgttg gttttttgg agccatgat ggagaatcg attcaaaat aacgaccga tatgagatc gaatacgtg gacaagtgt tccattttg aaaattgct gaagcgaca agaggtgat cgaggggag taccagatc aaagctgtg aacaagatt ggagaggat ttggcctcg tttttggtc accatcacc gacaagcct tcgccacca ggtcgggca agagtggtt atgaccttg ggccgttcc gtcaccctc tcctggagc acccctgac gatgatggg ggttgtaaa atcgggaac tacatcgta gagtactac agactgggg tggaacgtc tggctcaaa gctgctact agtagacaa ctgaccaca atcctaggg gacttaatc gaaggaagc gagtacaaa ttcaggatc aaggccgaa agtccgtac gggattagc gaaccgggg gctgaatcc gacatcgtc ttcatccct gatcccaaa agagggatc acttcgccc caagcgcga agccgaagc caaccgaag gatatcata gacgaaatc acttcagtc ccagttgcg gccaaacga cgtccaaaa cccaggtcg cagtcatca ataaccaaa gcagaaaca aattactat gacgagatg cccaaacgt ccagaacgt aacaaaatc aaaagtcct cccaaaaca ccagaagtt gtacgaaag aacgcaagt cctcaaact atcctcgat cgggcttcg ctagctcgg gaattggcg tacggaagt ccagaaatt aaaatgttg cagtcagaa aggccgacc agtcctttg ccaccacaa accaaaagc ccaagtccg ccaaaatcg cgaactccg agtcctaat ctcaccccg tctccctct gaagataag agcaagacc ccaagtccg aaagacaag aggagtttg cttcgggag aagtccgaa acgttcgaa gagagttcg gagtttatg ctggtctta tatccggat gctaacgaa aaaggtcgt cgtcggtcc agtcaaaac atcgaattc gactttgac gagtacgat atcccccct ccactctcc ctctcagct ccagagctc ggcgcagaa ccgcctgtt ttcgaaaac ttgaaaaca tcagcgagt tccaccgaa ctgttgcac gaacgcgcc atgatgcgc ttcaacgaa gccgcagcc gctgaagaa gaagagttg aagaaacga agaaagttc agttatgac ggccaacac aacatcgac atacccaaa atccaaatc aactcaaag gatgatcag gatatcgtc ggtctggaa cggaaacct tcccgaagg aaatcgtcg gggagtgtg gtccagcag cagctgctc tgggcccag aaacgcttc agtttgaag aattcgaac gatttgaat gaatttatc gaaacgaaa ttgcaaaaa acgcaaatt cctagtccc gaagcgccc aagaaggaa atggtgcgg caaagatcg gagtctgag gaacgcgag gaagaggaa tttaaccga gtgagggca aaaatgggg ctccaaggc cagtctagt ttggaaaaa cgcagcatt gaagtggtg gatgaagaa aagtggttg gaggattac gaagaaagt ttatcagag tcggaaact gaatctgaa gatgagcgg gattacgac atccaaccc cctcaaaga atctacagt gatgaagat gaagaaacg taccaccct ggcgtcatg accacgccc agaaaacca tcagttagt gacgaaccg tttgaaatt ttgaccaaa agaaacaaa ctacctgat ccgaacttc gtaccaaaa ccaattctg aagaaaact gaagtggaa ccaccgaaa gtttcaagt accaaaata aggtcacac tcgcccatg cctcagttt cggcaagaa gtgactaga gggcgtttg caatctttg gcacaacca ccaatagaa ttgtcggat aacaaaagc aaggttttc caacgctcg atttccctc tccactgaa gaaaacaca gaaagtgaa aaaatcgcc ccaacactt caggccgct ccaggacgg aatatttca gcagtggcg actctttgt ggtattacg gctgccagt atcgtcata ccggaaaaa ctgcttcag aagaaaaat gatgaagaa gagtcgaag gttgttgtg gaccactac atggacatt gtgaggagt tatggacaa cgaaagaag agtaatccc gaagtttgg aaagcggca acaaacgaa gcaaagacc tggagtacc accaaagaa gaaaaaact gaagaagaa gcacaaact ttgatgagt tatggccaa cgaaagaat agtaatccc caaattagg aaggtatta gaagaaaca aaaccagaa gctaaaacc tggagtacc aacaaagaa gaaaaagat gaggaacta acagagatt gtcaagagt tctggtcaa aggaagaat agtgttccc caaagtttg aaggaagaa gtgaagact tggaacacc accaaagaa gaaaaaagc gaagttcaa cctctcagg accgaacgt agtccctct ccctcccct caaaaaacc gctccgaac cagaaaaga gggagaagc ggcgtcaac cacaaacct aggagaggt tccagtcgt tccaaaacc ccctctcgt tccccttcc agactggaa aactggtca ggacgcaag tccagccct tcgcccatg aaacgcaaa acttccccc tctcccatg agggccagg aaatcacca tcgcccatc cctttggcc aaacctcgg cccaaacta cgtgaaatc acaactcag acgtccatc ggtttagaa ttgaactat tcgagcgat tctcgagct tccactcct cacgacagg agacaggag gagcttttg gctaaagct gaagtcaaa gttcgcagc tttgtcgat tatcttacg gatctggcg atgtttttc gtggcttgt tggttgtac ttgttcagt aatgaactc ttagcgatt cctgttttg ttggtcatg gtttacaga cagttgaag aatgagatc gggaaaagg ataccacga tggattgtt agacgattt agtaaaaag aaagcgaag aagtaa
- >TC002437 RA
caagttatt tttaacata gcacagtgt ggtgacggt tttgccgag atgatagtg tgcgatggg gaacacaca cgcgaagcc ccatccaaa accgaggaa taaaaaaca agtacactg gaaggctgc agaccgccc ctccccgcc cggcaagcc ccacctctc cgacaacga cctcacccc cgacctcgt caccatcgt ctggtctaa gccaacccg cgatggcgg ctctccaat caccggcta cctcgtcga gcaccgccg cacaggctc cccccactg ggtgcgagc cacgcccct cctcgtccc cttccccga gctcaccct cagcggcct ggagcccgg ctggcgcta ccagttccg ggtgcgggc cgaaaacgc agttggcct ttccgaacc cagcgacat ctccgaacc cttaactgt aacgttgca aaaattcgc tattacagc accaaagtt caccgaaga actcaaaga cgcaacagc gctcgaaaa tgagaaagt tgagttcgt tgtccactt tttgggaca acctgcgcc gaaaatctg ctggtttaa ggacgggtt tgagatttt tagcagcag aaggacgag gattttgac ggagaatga tcggagtgt gttgactat acaccagag cgcgctgtc ggatgaggg cgagatcaa gtgcacagc gaccaataa ggcggggca tgcgtccac gaaggcgcg gctaagctt ggaagcccc gcccagtat acgcctacc gagacaata cgaagatgg gctgttgtt tgagatcgg ggaagttat taggttgaa ggtttctgt ggctggccg tccgactcc gttggtttt ttggagcca tgatggaga atcgattca aaataacga ccgatatga gatcgaata cgtggacaa gtgttccat tttgaaaat tgctgaagc gacaagagg tgatcgagg ggagtacca gatcaaagc tgtgaacaa gattggaga ggatttggc ctcgttttt ggtcaccat caccgacaa gccttcgcc accaggtcg ggcaagagt ggttatgac cttgggccg ttccgtcac cctctcctg gagcacccc tgacgatga tgggggttg taaaatcgg gaactacat cgtagagta ctacagact ggggtggaa cgtctggct caaagctgc tactagtag acaactgac cacaatcct aggggactt aatcgaagg aagcgagta caaattcag gatcaaggc cgaaagtcc gtacgggat tagcgaacc gggggctga atccgacat cgtcttcat ccctgatcc caaaagagg gatcacttc gccccaagc gcgaagccg aagccaacc gaaggatat catagacga aatcacttc agtcccagt tgcggccaa acgacgtcc aaaacccag gtcgcagtc atcaataac caaagcaga aacaaatta ctatgacga gatgcccaa acgtccaga acgtaacaa aatcaaaag tcctcccaa aacaccaga agttgtacg aaagaacgc aagtcctca aactatcct cgatcgggc ttcgctagc tcgggaatt ggcgtacgg aagtccaga aattaaaat gttgcagtc agaaaggcc gaccagtcc tttgccacc acaaaccaa aagcccaag tccgccaaa atcgcgaac tccgagtcc taatctcac cccgtctcc ctctgaaga taagagcaa gaccccaag tccgaaaga caagaggag tttgcttcg ggagaagtc cgaaacgtt cgaagagag ttcggagtt tatgctggt cttatatcc ggatgctaa cgaaaaagg tcgtcgtcg gtccagtca aaacatcga attcgactt tgacgagta cgatatccc ccctccact ctccctctc agctccaga gctcggcgc agaaccgcc tgttttcga aaacttgaa aacatcagc gagttccac cgaactgtt gcacgaacg cgccatgat gcgcttcaa cgaagccgc agccgctga agaagaaga gttgaagaa acgaagaaa gttcagtta tgacggcca acacaacat cgacatacc caaaatcca aatcaactc aaaggatga tcaggatat cgtcggtct ggaacggaa accttcccg aaggaaatc gtcggggag tgtggtcca gcagcagct gctctgggc ccagaaacg cttcagttt gaagaattc gaacgattt gaatgaatt tatcgaaac gaaattgca aaaaacgca aattcctag tcccgaagc gcccaagaa ggaaatggt gcggcaaag atcggagtc tgaggaacg cgaggaaga ggaatttaa ccgagtgag ggcaaaaat ggggctcca aggccagtc tagtttgga aaaacgcag cattgaagt ggtggatga agaaaagtg gttggagga ttacgaaga aagtttatc agagtcgga aactgaatc tgaagatga gcgggatta cgacatcca accccctca aagaatcta cagtgatga agatgaaga aacgtacca ccctggcgt catgaccac gcccagaaa accatcagt tagtgacga accgtttga aattttgac caaaagaaa caaactacc tgatccgaa cttcgtacc aaaaccaat tctgaagaa aactgaagt ggaaccacc gaaagtttc aagtaccaa aataaggtc acactcgcc catgcctca gtttcggca agaagtgac tagagggcg tttgcaatc tttggcaca accaccaat agaattgtc ggataacaa aagcaaggt tttccaacg ctcgatttc cctctccac tgaagaaaa cacagaaag tgaaaaaat cgccccaac acttcaggc cgctccagg acggaatat ttcagcagt ggcgactct ttgtggtat tacggctgc cagtatcgt cataccgga aaaactgct tcagaagaa aaatgatga agaagagtc gaaggttgt tgtggacca ctacatgga cattgtgag gagttatgg acaacgaaa gaagagtaa tcccgaagt ttggaaagc ggcaacaaa cgaagcaaa gacctggag taccaccaa agaagaaaa aactgaaga agaagcaca aactttgat gagttatgg ccaacgaaa gaatagtaa tccccaaat taggaaggt attagaaga aacaaaacc agaagctaa aacctggag taccaacaa agaagaaaa agatgagga actaacaga gattgtcaa gagttctgg tcaaaggaa gaatagtgt tccccaaag tttgaagga agaagtgaa gacttggaa caccaccaa agaagaaaa aagcgaagt tcaacctct caggaccga acgtagtcc ctctccctc ccctcaaaa aaccgctcc gaaccagaa aagagggag aagcggcgt caaccacaa acctaggag aggttccag tcgttccaa aaccccctc tcgttcccc ttccagact ggaaaactg gtcaggacg caagtccag cccttcgcc catgaaacg caaaacttc cccctctcc catgagggc caggaaatc accatcgcc catcccttt ggccaaacc tcggcccaa actacgtga aatcacaac tcagacgtc catcggttt agaattgaa ctattcgag cgattctcg agcttccac tcctcacga caggagaca ggaggagct tttggctaa agctgaagt caaagttcg cagctttgt cgattatct tacggatct ggcgatgtt tttcgtggc ttgttggtt gtacttgtt cagtaatga actcttagc gattcctgt tttgttggt catggttta cagacagtt gaagaatga gatcgggaa aaggatacc acgatggat tgttagacg atttagtaa aaagaaagc gaagaagta ataacatgt actgtttgc acggttttt ttctattgt ttgtgtgag attttccga acacgacgt tatgaaatc tatgatttc acttatctt atttaggca gtgattgtg tgtactttc aattaagtg gtgtacata atttttatt aagcgaaac gttatggtt tcaccggcc tttaaataa ataacataa atccca
If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.
