| General information | |||
| TC001512 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007425.3: 2454263..2516083 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC001512 | |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
| Gene | {{ goGene }} |
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| GO ID | * {{ blurIdResult }} |
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| Evidence | {{ blurEvidenceResult }} |
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| Reference | * {{ blurReferenceResult }} |
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| Quotation | ||
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| Publish name | ||
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_03200 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
||
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)
- >TC001512 RA
MLSEFVYLF PLLFLLPRT KSDNLPFRP ISKPLTFPK ENRLNEFIR HYEPLSYDT SEVHRAHNR ARRSVSRDN LVHVSFRAH NRDFHLRLK RDLDVFSDS MEVHGPEGR IDVDTSHVY KGHLLGEPE SSVFGSLID GVFEGKIVS PKGSFYVEK ARHYFPHTT HPNRTFHSV IYHEDHVED PYEHVREGH SAGCGITDE VIQWMDRVQ NSGVDDEPP PPPPKETFR KRKKATNGD KVQFKSVEN HEDQISKYS KEANEDHAR HKRATRRDE NKNTCSLYI QTDPLIWRH IREGFPEHS DPRKENEVN EKTREEILS LIAHHVTAV NYIYRDTKF DGRSEHRNI KFEVQRIKI DDYSSCSCK RCPEMNQFC LENIDVSNF LNIHSLGNH EDFCLAYVF TYRDFTGGT LGLAWVASA SGASGGICE RYKTYTETI GGMYQSTKR SLNTGIITF VNYNSRVPP KVSQLTLAH EIGHNFGSP HDYPPECRP GGLNGNFIM FASATSGDR PNNSKFSSC SVGNISNVL DAIEDNKKR NCFTASAGA FCGNKIVEA GEECDCGYD DNECQDKCC YPRLVSTQD KQENASAKG CQRRRGTQC SPSQGPCCS GDTCRFVPA YYGEVCKTE SDCSMSSKC DGKSAECPQ PQPRPDKTR CNNGTQLCI KGECTGSIC LEWNLQACS ITSQGNPNI DKRKLCELA CQNGTDMCR STSEFAEKV GLPPGGISL RPGAPCDNF QGYCDVFLK CRAVDADGP LVRLMNLLL NKETLLSVA QWITNYWWA VLLMGIAFI IFMGLFIKC CAVHTPSSN PKKRPARRI SDTLRRPMN TLRRMRHHQ NGAQHAAPH GAVNSRNRS ERSTRPSAS QGPRPGYGE GRGHYHAPK ATAPPSGFN RQNHPYAGA YDRNAYEMR HKV
- >TC001512 RA
atgttgagt gaatttgtt tacttgttc cctttgctt ttcttgctt cctcgaacc aaaagtgat aatttgccc ttcagaccc atcagtaaa ccgctgaca tttccaaaa gaaaaccgc ctcaacgag ttcatccgc cactacgag cctctatcg tacgacacc tccgaagtg catcgagcc cacaaccgt gctcgtcgt tctgtcagt cgcgataat ctcgtccac gtgtcgttc cgagctcac aaccgtgat ttccatcta agactgaaa agagacctt gatgtgttc agcgattcg atggaggtt cacggtccc gaaggccgg atagacgtc gatacgtcg cacgtctac aaaggccac cttttaggt gagcccgag agttcggtc tttggctcg ttaattgat ggagtcttt gaagggaag atcgtatca cctaaaggc tctttttac gttgagaag gctcgtcat tattttccc cataccaca catccaaac agaacattc cattcggtc atttaccac gaggatcac gtagaggat ccatacgaa catgttaga gagggtcat tcagctgga tgcggaatc accgacgag gtgatccag tggatggac cgcgtccag aattcgggg gtggacgac gagccgccg ccgccgccg cccaaagaa acattccga aaacgtaaa aaagccacc aatggtgat aaagttcaa ttcaagtcc gtcgaaaat cacgaagac cagatcagc aagtattcg aaagaggcg aatgaagat cacgcaaga cataaaagg gcaacacga agggacgaa aacaagaac acttgttcg ttgtatatc cagacggat ccactcatc tggagacac atcagggag ggctttcca gagcactct gacccgagg aaggaaaac gaagtcaac gaaaagaca cgcgaagaa atcctgtca ttaatcgca catcacgtc actgccgtt aattatatt tatcgtgat acgaaattt gatgggaga agcgaacat cgcaatatc aaatttgaa gttcaaagg attaagatc gacgactat tcctcctgc agctgcaag cgatgcccc gaaatgaac cagttttgt ttagaaaat atcgacgtt agtaatttc ttgaatatc cattcgttg ggcaatcac gaggacttt tgtctggct tatgttttc acgtatcgg gattttacg ggtggtact ctggggcta gcttgggtc gctagtgcg tccggggct tccggcggt atctgcgag agatacaag acttacacg gaaacaata ggaggcatg tatcagtcg acgaagcgc tccttaaac acagggatc atcactttc gtcaactac aacagcagg gtgcctcct aaggtctcc cagctcaca ctagcgcac gaaatcgga cacaatttc ggctctccc cacgactat ccgcccgaa tgccgtccg ggtggcctt aacggcaac ttcataatg ttcgcgtcg gcaacaagc ggcgaccgc cccaacaac agcaaattt tcaagttgt agcgtcggc aacatctcc aatgtgctc gacgccatc gaagacaat aaaaagcgc aattgtttc accgcatcg gctggtgct ttctgtggc aataaaatc gtcgaagcc ggcgaagag tgtgattgt ggttacgac gacaacgag tgtcaagat aagtgttgc taccctcgt cttgtcagc actcaagac aagcaagaa aacgcttca gcgaaaggc tgtcagagg cgaagaggc acccaatgc agtcctagt caaggcccg tgctgcagt ggcgacacc tgccgcttc gtcccagcc tactacggc gaggtttgc aaaaccgag tcggattgt tcgatgtcg tcgaaatgc gacgggaaa tcggctgag tgcccacag ccccaacca cggccggat aagactagg tgcaataat gggacacag ttgtgtatt aagggggag tgcacgggg tccatttgt ctggaatgg aacctacaa gcttgttcg attacctcg caaggtaat ccaaacatc gataagaga aaactgtgc gagttggct tgtcagaac ggaacggac atgtgtcga agtacgagc gaattcgct gagaaagtc gggcttcca ccaggtggg atcagccta aggccggga gcgccgtgc gataatttt cagggttac tgtgacgta ttcctcaaa tgccgagcc gtcgacgct gacggcccc ctagtcaga ttgatgaat cttctattg aacaaagag acgctgctc agcgtcgcc caatggata acgaattac tggtgggcg gtgctgttg atgggcatc gcgtttatc attttcatg ggtttgttt atcaaatgt tgcgccgta catacgccg tcttccaat ccgaagaaa cggccggcg agacgtatc agcgatacg ctccgcaga cccatgaac acgctccgg cgaatgcga caccaccaa aacggggcg caacatgcg gcgccccat ggggctgta aatagtcga aatcgcagt gaaaggtcc acgagaccg agcgctagt caagggccc aggccgggc tacggggag ggaagggga cactatcat gcgccgaaa gcgacagcg ccgccttcc ggtttcaac cgccaaaac cacccatac gcaggggcg tacgaccgt aacgcctac gagatgcgt cacaaagtt tga
- >TC001512 RA
aatgaatcg gttttacag taaactggt ttttattaa attccaagt ggccaaaga cttgtcgtt tcagtttcg acttggtgg cccccttgt tgacatgtc tcagtcacc catcaataa ttctcgtta atttggtca aaatttgta aaataaaac agcaacgca tgcgcagtg gcgtctaaa gacaaaggt gttgtgtac aaaccagtg catcagtca gctgtaaat tcaggaatg gcttagttg gggtgtgtt tttgtggtg tttgtggga ttattttgt gcaggaaaa tgttgagtg aatttgttt acttgttcc ctttgcttt tcttgcttc ctcgaacca aaagtgata atttgccct tcagaccca tcagtaaac cgctgacat ttccaaaag aaaaccgcc tcaacgagt tcatccgcc actacgagc ctctatcgt acgacacct ccgaagtgc atcgagccc acaaccgtg ctcgtcgtt ctgtcagtc gcgataatc tcgtccacg tgtcgttcc gagctcaca accgtgatt tccatctaa gactgaaaa gagaccttg atgtgttca gcgattcga tggaggttc acggtcccg aaggccgga tagacgtcg atacgtcgc acgtctaca aaggccacc ttttaggtg agcccgaga gttcggtct ttggctcgt taattgatg gagtctttg aagggaaga tcgtatcac ctaaaggct ctttttacg ttgagaagg ctcgtcatt attttcccc ataccacac atccaaaca gaacattcc attcggtca tttaccacg aggatcacg tagaggatc catacgaac atgttagag agggtcatt cagctggat gcggaatca ccgacgagg tgatccagt ggatggacc gcgtccaga attcggggg tggacgacg agccgccgc cgccgccgc ccaaagaaa cattccgaa aacgtaaaa aagccacca atggtgata aagttcaat tcaagtccg tcgaaaatc acgaagacc agatcagca agtattcga aagaggcga atgaagatc acgcaagac ataaaaggg caacacgaa gggacgaaa acaagaaca cttgttcgt tgtatatcc agacggatc cactcatct ggagacaca tcagggagg gctttccag agcactctg acccgagga aggaaaacg aagtcaacg aaaagacac gcgaagaaa tcctgtcat taatcgcac atcacgtca ctgccgtta attatattt atcgtgata cgaaatttg atgggagaa gcgaacatc gcaatatca aatttgaag ttcaaagga ttaagatcg acgactatt cctcctgca gctgcaagc gatgccccg aaatgaacc agttttgtt tagaaaata tcgacgtta gtaatttct tgaatatcc attcgttgg gcaatcacg aggactttt gtctggctt atgttttca cgtatcggg attttacgg gtggtactc tggggctag cttgggtcg ctagtgcgt ccggggctt ccggcggta tctgcgaga gatacaaga cttacacgg aaacaatag gaggcatgt atcagtcga cgaagcgct ccttaaaca cagggatca tcactttcg tcaactaca acagcaggg tgcctccta aggtctccc agctcacac tagcgcacg aaatcggac acaatttcg gctctcccc acgactatc cgcccgaat gccgtccgg gtggcctta acggcaact tcataatgt tcgcgtcgg caacaagcg gcgaccgcc ccaacaaca gcaaatttt caagttgta gcgtcggca acatctcca atgtgctcg acgccatcg aagacaata aaaagcgca attgtttca ccgcatcgg ctggtgctt tctgtggca ataaaatcg tcgaagccg gcgaagagt gtgattgtg gttacgacg acaacgagt gtcaagata agtgttgct accctcgtc ttgtcagca ctcaagaca agcaagaaa acgcttcag cgaaaggct gtcagaggc gaagaggca cccaatgca gtcctagtc aaggcccgt gctgcagtg gcgacacct gccgcttcg tcccagcct actacggcg aggtttgca aaaccgagt cggattgtt cgatgtcgt cgaaatgcg acgggaaat cggctgagt gcccacagc cccaaccac ggccggata agactaggt gcaataatg ggacacagt tgtgtatta agggggagt gcacggggt ccatttgtc tggaatgga acctacaag cttgttcga ttacctcgc aaggtaatc caaacatcg ataagagaa aactgtgcg agttggctt gtcagaacg gaacggaca tgtgtcgaa gtacgagcg aattcgctg agaaagtcg ggcttccac caggtggga tcagcctaa ggccgggag cgccgtgcg ataattttc agggttact gtgacgtat tcctcaaat gccgagccg tcgacgctg acggccccc tagtcagat tgatgaatc ttctattga acaaagaga cgctgctca gcgtcgccc aatggataa cgaattact ggtgggcgg tgctgttga tgggcatcg cgtttatca ttttcatgg gtttgttta tcaaatgtt gcgccgtac atacgccgt cttccaatc cgaagaaac ggccggcga gacgtatca gcgatacgc tccgcagac ccatgaaca cgctccggc gaatgcgac accaccaaa acggggcgc aacatgcgg cgccccatg gggctgtaa atagtcgaa atcgcagtg aaaggtcca cgagaccga gcgctagtc aagggccca ggccgggct acggggagg gaaggggac actatcatg cgccgaaag cgacagcgc cgccttccg gtttcaacc gccaaaacc acccatacg caggggcgt acgaccgta acgcctacg agatgcgtc acaaagttt gacagaacg gtcaagtcg gggcaattc gggtgtctc cgccgcccg attaccccg gcacaccaa tggccaaca tacatttcg agggtccca tggcacaat cgtcaaacc agcaaaaga acactagtg tacagagtg gctcaactt aacaaactc gaaacgatt gttgtgctc tgtgatcga ttcgaacca cgaaccaat aaaaagtgc gtgtgatat tacgaaact cgaaatgat tgattgaaa acgggtgca tttatgtat agtcaccta ggttttagg gatgagttt ttatgatat tagttggct tccgacgaa aggctgaac ggatttgcg gttttatct gccaactcg attttttaa tttatttat ttgccattt ctgcaccaa atgattagg ttttaggtt cacgtacat atccgaaca aatttgtcg atcaaagtt tttgattat gggggaatt gaattggat tttgattgt ggattcggt ttgtccata attccaagt ggttacatc acagctgcg ttttttagt aaaattgtg gaaatttcg tgcagagat ctcattttg aatgatttg tcctgaaag ttgctattt ttatataat tgagggacg caattttac atttggtac attgttgaa tttcaggaa attgtgata caaatctaa tttgctgtt ttgtaatgt ctcaatgga gaaatctat atttgatgc tgaaaatct gtattaatg aattgtaaa atattgata tattatgta aatattgga aaaacatag ttgttttta tatgaagta tgtatcata ttcttcact tttgtttta gagtagtgg attttacct atgtctagc atttgtttg attttttga cgacgtaaa agtaattgt atcaaattt ttttattat gctagtagg tacaatcca acctcgatg accaataat tgttgtgca tgtcgctta atacatgta ccaaagtca ttggatgac tttttataa tactgtgaa taataaatg tgttattgt caa
If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.
