Status
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Gene information
General information
TC000956 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 8866505..8869816
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC000956
    Gene ontology

    GO terms for Tribolium

    • {{ goTerm.term }}
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      Status: {{ goTerm.status }}
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      Quotation: {{ goTerm.quotation }}
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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
    {{ blurIdResult }}
    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
      {{ unavailableText }}

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_00196

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 4 (9 dpi)
    Days post injection


    Fertility
    vitellogenic egg chamber mostly degenerated - Number of animals showing the phenotype: 4

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 25.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    pupal molt delayed (still larva) - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    larva lethality late (L7) - Number of animals showing the phenotype: 3 (16 dpi)
    Days post injection


    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 2 (16 dpi)
    Days post injection


    Pupal morphology
    mesonotum central colour darker - Number of animals showing the phenotype: 2

    iBeetle screen iB_00196

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: % (includes death as pupa, adult)

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC000956 RA
      MSDSESQTW LGTPLSDMY KGQGPWGFA TAPVTPAKY 
      HHVLFELPV TLKEPPEPH YNAQPKHWD DDYVRMPYS 
      SHSLFLVKE NGEEVIKPR WDVINEALS KPITSVEDL 
      EKAINTYNN KLPKFTALK HFFNDVIEE EEAQLFFTS 
      LLPKIIKLA LRLPELVPG SIPLLRKNH SKSVSLSQL 
      QVASLIANA FLCTLIWRK KTSDTFPGV NFTKLFNAD 
      DRTLRQECV AEKLKCIMH YFNRVTTKT PVGVITFER 
      KFVPRSQMP RWDALDNNL GNTKVHISS SGTIEEARG 
      FLQVDFANK FIGGGVLSY GCVQEEIRF VICPELIIS 
      RLFTEVLGD GEAVIITGI ERYSNYSGY SNTFKWDGN 
      VNDDTPFDD FGRRKTTLA VIDATQFTK SKDQFHCST 
      ILRELNKAY VGFSCREKQ NLAPVATGN WGCGAFKGD 
      PNLKSLIQL MACSASGRD LVYYSFGDV ELRDEFYKM 
      YMFLANNKI TICQLWRYL CRFCGANVT PDKLYSFIQ 
      QCHFDSKTQ PTIKQFFGL TQKRKPDLN LPSTSAEDA 
      VKTKKTSPN ERKVKDEAK PSVAKYPEL PVTIERKKS 
      PVTPDEIIE IIDIIDGNV AKSRDNAEQ EEESLLSKI 
      DKMKKSETK KSDDCEIPM EVDVEPKPV GFIEDKKRK 
      ISDYFSKVT NHVNSYL
    CDS sequence
    • >TC000956 RA
      atgagcgat agtgaatcc caaacatgg ctaggtacc 
      cccttgtcg gacatgtac aagggccag gggccgtgg 
      ggcttcgcc actgctcct gtcaccccc gcgaagtat 
      caccacgtt ctattcgag ctccctgtg acgctgaag 
      gagcccccg gagccccat tataacgca caacccaag 
      cattgggat gacgattac gtccggatg ccttactcg 
      tcccatagc ttatttcta gtgaaagaa aatggagaa 
      gaagtgata aaaccgcgc tgggacgtc ataaacgaa 
      gccctttcg aaacctatc acctcagtc gaagattta 
      gaaaaagca ataaataca tataacaac aaactacca 
      aaatttaca gcactgaaa cattttttt aatgatgtt 
      atagaagag gaggaagcg cagcttttt ttcacgtcc 
      ctgttacca aaaatcatc aaacttgcg ttacgtctg 
      cctgagtta gtccctgga agtattcca ctgttgcgg 
      aaaaatcat tccaaatcg gtcagtctg agccagtta 
      caagtggct tcgctgatt gcgaatgcg tttttgtgc 
      actctgatc tggaggaaa aaaacgtcc gatactttc 
      cccggcgtt aatttcaca aaattattt aacgccgat 
      gatcggacg ttacgccag gaatgtgtc gcggaaaaa 
      ttaaagtgc atcatgcac tactttaat cgagtcacc 
      accaaaact ccagtcggt gtcataaca ttcgagcgc 
      aagttcgtc cccaggagc caaatgcca cgatgggac 
      gctctggac aataattta ggaaacacc aaagtgcat 
      atctcaagc tcagggacg atagaagag gctcgaggt 
      tttttacaa gtcgatttt gcgaacaag tttataggc 
      ggcggtgtt ctgtcgtat ggctgtgtt caggaggag 
      attcggttt gtcatttgt cccgaattg ataatcagc 
      cgcctgttc accgaggtg ttgggtgac ggcgaagcc 
      gtcattatc accggaatt gagcgatat agcaattat 
      tccggttat tccaacacc ttcaagtgg gacggaaac 
      gtcaatgac gacacaccg tttgatgat ttcggccga 
      cgaaaaaca acgttggct gtgatagat gcgactcaa 
      ttcacaaaa tccaaagac cagttccac tgttcgacc 
      atattacgc gagctcaat aaagcttat gtggggttt 
      agttgtcgc gaaaagcaa aacttggcg cctgttgcg 
      acaggcaat tggggctgt ggggccttc aaaggcgac 
      cccaactta aaatcattg atccaactc atggcatgt 
      agcgcctct ggacgcgac ttggtctac tactcgttt 
      ggcgatgtc gagctccga gatgaattt tacaaaatg 
      tatatgttc ctcgccaat aacaaaatc actatttgc 
      caactgtgg cgttatttg tgccgcttt tgtggcgca 
      aacgtcact ccagacaag ttatactcc tttatccaa 
      caatgccac ttcgattcc aagacacag ccaacaata 
      aaacaattt tttgggctc acacagaag cggaaaccc 
      gatctcaat ttgccgtca acgtcggcc gaagatgca 
      gttaaaacg aagaaaact tcgccgaac gagcggaaa 
      gttaaggac gaggcgaag ccgagtgtt gcgaaatat 
      cccgagtta ccggtgaca atcgaacgg aagaaaagt 
      ccagtcact cccgacgaa attatcgag attatagat 
      atcatagac gggaatgtg gcgaaaagt cgggacaat 
      gccgagcaa gaggaggaa agtctcctg tccaagatc 
      gacaaaatg aagaaaagc gaaacgaag aagagcgac 
      gattgcgag atcccgatg gaagtggat gtggagccg 
      aaacccgtg gggttcatc gaagacaag aagaggaaa 
      atcagcgat tatttctcc aaagtgacc aatcatgtc 
      aacagctat ttgtaa
    mRNA sequence
    • >TC000956 RA
      tcagttttg acgtttctc tgcctaacc taacttttg 
      ccaataata ataaatatg aagcgccaa tttatgtag 
      tgacaatga gcgatagtg aatcccaaa catggctag 
      gtaccccct tgtcggaca tgtacaagg gccaggggc 
      cgtggggct tcgccactg ctcctgtca cccccgcga 
      agtatcacc acgttctat tcgagctcc ctgtgacgc 
      tgaaggagc ccccggagc cccattata acgcacaac 
      ccaagcatt gggatgacg attacgtcc ggatgcctt 
      actcgtccc atagcttat ttctagtga aagaaaatg 
      gagaagaag tgataaaac cgcgctggg acgtcataa 
      acgaagccc tttcgaaac ctatcacct cagtcgaag 
      atttagaaa aagcaataa atacatata acaacaaac 
      taccaaaat ttacagcac tgaaacatt tttttaatg 
      atgttatag aagaggagg aagcgcagc tttttttca 
      cgtccctgt taccaaaaa tcatcaaac ttgcgttac 
      gtctgcctg agttagtcc ctggaagta ttccactgt 
      tgcggaaaa atcattcca aatcggtca gtctgagcc 
      agttacaag tggcttcgc tgattgcga atgcgtttt 
      tgtgcactc tgatctgga ggaaaaaaa cgtccgata 
      ctttccccg gcgttaatt tcacaaaat tatttaacg 
      ccgatgatc ggacgttac gccaggaat gtgtcgcgg 
      aaaaattaa agtgcatca tgcactact ttaatcgag 
      tcaccacca aaactccag tcggtgtca taacattcg 
      agcgcaagt tcgtcccca ggagccaaa tgccacgat 
      gggacgctc tggacaata atttaggaa acaccaaag 
      tgcatatct caagctcag ggacgatag aagaggctc 
      gaggttttt tacaagtcg attttgcga acaagttta 
      taggcggcg gtgttctgt cgtatggct gtgttcagg 
      aggagattc ggtttgtca tttgtcccg aattgataa 
      tcagccgcc tgttcaccg aggtgttgg gtgacggcg 
      aagccgtca ttatcaccg gaattgagc gatatagca 
      attattccg gttattcca acaccttca agtgggacg 
      gaaacgtca atgacgaca caccgtttg atgatttcg 
      gccgacgaa aaacaacgt tggctgtga tagatgcga 
      ctcaattca caaaatcca aagaccagt tccactgtt 
      cgaccatat tacgcgagc tcaataaag cttatgtgg 
      ggtttagtt gtcgcgaaa agcaaaact tggcgcctg 
      ttgcgacag gcaattggg gctgtgggg ccttcaaag 
      gcgacccca acttaaaat cattgatcc aactcatgg 
      catgtagcg cctctggac gcgacttgg tctactact 
      cgtttggcg atgtcgagc tccgagatg aattttaca 
      aaatgtata tgttcctcg ccaataaca aaatcacta 
      tttgccaac tgtggcgtt atttgtgcc gcttttgtg 
      gcgcaaacg tcactccag acaagttat actccttta 
      tccaacaat gccacttcg attccaaga cacagccaa 
      caataaaac aattttttg ggctcacac agaagcgga 
      aacccgatc tcaatttgc cgtcaacgt cggccgaag 
      atgcagtta aaacgaaga aaacttcgc cgaacgagc 
      ggaaagtta aggacgagg cgaagccga gtgttgcga 
      aatatcccg agttaccgg tgacaatcg aacggaaga 
      aaagtccag tcactcccg acgaaatta tcgagatta 
      tagatatca tagacggga atgtggcga aaagtcggg 
      acaatgccg agcaagagg aggaaagtc tcctgtcca 
      agatcgaca aaatgaaga aaagcgaaa cgaagaaga 
      gcgacgatt gcgagatcc cgatggaag tggatgtgg 
      agccgaaac ccgtggggt tcatcgaag acaagaaga 
      ggaaaatca gcgattatt tctccaaag tgaccaatc 
      atgtcaaca gctatttgt aatttcttg aataaattt 
      ttgatttat tt

    If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
    Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.

    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY