Status
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Gene information
General information
TC000944 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 8680200..8683282
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC000944
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_00193

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    remarks: later than schedule, hours 100
    Adult morphology
    remarks: later than schedule, hours 100
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC000944 RA
      MLSLVSCLC FLPLIASSY KSPHFSPNR STIVHLFEW 
      KWSSIASEC ENFLQHKAY AGVQVSPPN ENLIIDGRP 
      WWERYQPVS YVLITRSGN ASSFADMVK RCNAVGVRI 
      YVDAVINHM SAGNGVGTG GSLANASHK FYPGVPYGP 
      DDFNKNCEI TNFDNNENA RNCELVGLK DLNQSKKYV 
      KGKIIEYLN HLIDLGVAG FRIDAARHM WPSDLREIY 
      SNLKNLNTE FGFAENLRP FIYQEVPTM DKEYTKLGA 
      LVEFNYGKS LSNVFRGND KLKWLVNWG PSWGLIDGS 
      DAVAFIDNH DTQRDKNGH ILSHKKAKQ YKMAVAFML 
      AHPYGTTRI ISSYAFNDF NQGPPADKT GDLIPPNFN 
      KDNTCGNGY VCEHRWREI YSMVQFRNV VAGTGVENF 
      WSNDDQQIS FCRGNKGFI VFTNWGDVE RDLQTCLPP 
      GIYCDVISG SLKAGNCTG KAVRVKSDG KGFIRLGAL 
      EFNGVLTIH EKSKLK
    CDS sequence
    • >TC000944 RA
      atgttaagc cttgtgtcg tgtctgtgt tttctcccc 
      ttgatagcc tcctcgtac aaatcgcct catttctcc 
      cccaaccgc tccaccatc gtccaccta ttcgaatgg 
      aaatggtcg agcatcgcc tccgaatgc gaaaacttc 
      ctccagcac aaagcctac gccggcgtt caggtttca 
      cccccgaac gaaaactta ataattgac ggccgacct 
      tggtgggaa agataccag cctgtgagc tacgtattg 
      attacgagg tccggtaac gcgagttct ttcgctgat 
      atggtcaag agatgcaac gctgtgggc gtgagaatt 
      tacgttgat gctgttatt aatcacatg tcggcggga 
      aatggggtc gggaccggt ggcagcctg gcaaatgcc 
      tcgcataaa ttttaccca ggagtgcca tacggcccc 
      gatgatttc aataaaaat tgcgaaata accaacttt 
      gataataac gaaaatgca agaaactgc gaactggtc 
      ggtctgaaa gacttgaat cagagcaag aaatacgtg 
      aagggcaaa ataatcgaa tacttgaat catttaatc 
      gacctgggg gtggcgggg ttccgaatc gacgccgca 
      cgacacatg tggccctca gacttgaga gaaatctac 
      tcgaatttg aaaaatcta aacactgag tttggcttt 
      gccgaaaat ttgagaccg tttatttac caggaggtg 
      ccaacaatg gacaaggaa tatacaaaa ttgggagct 
      ttggtcgag ttcaattat gggaaatcg ttaagtaat 
      gtgtttaga ggcaatgat aaattaaaa tggttggtc 
      aattggggc ccatcgtgg ggtctaatc gatggttca 
      gacgctgtt gcgtttatt gacaaccac gacacccag 
      cgggacaaa aatgggcac attttgagc cacaaaaaa 
      gcaaagcag tacaagatg gcagttgcg tttatgttg 
      gcacaccca tatggtacg acccggatt atttcgagt 
      tatgccttc aatgatttc aatcaaggt cccccggct 
      gataagacc ggtgattta attcctcca aactttaat 
      aaggataac acttgtggc aatggttac gtctgcgag 
      catcgctgg agagaaatc tacagcatg gtgcaattc 
      cggaacgtc gttgctggg accggtgtt gaaaatttc 
      tggtcgaac gatgatcag caaatatcg ttctgtcgc 
      ggaaataag ggttttata gtttttact aattggggc 
      gatgttgag cgggattta cagacgtgt ctacctccc 
      gggatttac tgtgatgtt atatcagga agtctgaag 
      gccggaaat tgcaccggg aaagccgtt agagtaaaa 
      agtgacggc aaagggttt attcggctg ggggcgctt 
      gagtttaat ggtgtcctg acgatacac gaaaagtca 
      aaattgaaa taa
    mRNA sequence
    • >TC000944 RA
      cgttctcac aagatgtta agccttgtg tcgtgtctg 
      tgttttctc cccttgata gcctcctcg tacaaatcg 
      cctcatttc tcccccaac cgctccacc atcgtccac 
      ctattcgaa tggaaatgg tcgagcatc gcctccgaa 
      tgcgaaaac ttcctccag cacaaagcc tacgccggc 
      gttcaggtt tcacccccg aacgaaaac ttaataatt 
      gacggccga ccttggtgg gaaagatac cagcctgtg 
      agctacgta ttgattacg aggtccggt aacgcgagt 
      tctttcgct gatatggtc aagagatgc aacgctgtg 
      ggcgtgaga atttacgtt gatgctgtt attaatcac 
      atgtcggcg ggaaatggg gtcgggacc ggtggcagc 
      ctggcaaat gcctcgcat aaattttac ccaggagtg 
      ccatacggc cccgatgat ttcaataaa aattgcgaa 
      ataaccaac tttgataat aacgaaaat gcaagaaac 
      tgcgaactg gtcggtctg aaagacttg aatcagagc 
      aagaaatac gtgaagggc aaaataatc gaatacttg 
      aatcattta atcgacctg ggggtggcg gggttccga 
      atcgacgcc gcacgacac atgtggccc tcagacttg 
      agagaaatc tactcgaat ttgaaaaat ctaaacact 
      gagtttggc tttgccgaa aatttgaga ccgtttatt 
      taccaggag gtgccaaca atggacaag gaatataca 
      aaattggga gctttggtc gagttcaat tatgggaaa 
      tcgttaagt aatgtgttt agaggcaat gataaatta 
      aaatggttg gtcaattgg ggcccatcg tggggtcta 
      atcgatggt tcagacgct gttgcgttt attgacaac 
      cacgacacc cagcgggac aaaaatggg cacattttg 
      agccacaaa aaagcaaag cagtacaag atggcagtt 
      gcgtttatg ttggcacac ccatatggt acgacccgg 
      attatttcg agttatgcc ttcaatgat ttcaatcaa 
      ggtcccccg gctgataag accggtgat ttaattcct 
      ccaaacttt aataaggat aacacttgt ggcaatggt 
      tacgtctgc gagcatcgc tggagagaa atctacagc 
      atggtgcaa ttccggaac gtcgttgct gggaccggt 
      gttgaaaat ttctggtcg aacgatgat cagcaaata 
      tcgttctgt cgcggaaat aagggtttt atagttttt 
      actaattgg ggcgatgtt gagcgggat ttacagacg 
      tgtctacct cccgggatt tactgtgat gttatatca 
      ggaagtctg aaggccgga aattgcacc gggaaagcc 
      gttagagta aaaagtgac ggcaaaggg tttattcgg 
      ctgggggcg cttgagttt aatggtgtc ctgacgata 
      cacgaaaag tcaaaattg aaataattt agtcaaatg 
      aattttaat aaaattgcc cggcgctga agttctata 
      catatcaaa tcaacacaa acgtttgga ttaaatcga 
      aaaataaat tatttgtac gctccaaaa ttgtaaaac 
      aggaataaa taaaattat taatgcgcc ggcaaggtc 
      aatacagcc gaccataac gt

    If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
    Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.

    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY