Status
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Gene information
General information
TC000505 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 10512946..10515298
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC000505
    Gene ontology

    GO terms for Tribolium

    • {{ goTerm.term }}
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      Status: {{ goTerm.status }}
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      Quotation: {{ goTerm.quotation }}
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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
    {{ blurIdResult }}
    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
      {{ unavailableText }}

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_03033

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    iBeetle screen iB_03033

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: % (includes death as pupa, adult)

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC000505 RA
      MGDENRIRS FKNKGKDCE EMRRRRAGQ TIELRKAKK 
      EDQLLKRRN ISLQEEAST PLQENNSVS LITMSAEEI 
      LMGMMSPDE NLQFKATQA CRKMLSRER NPPIDHMIR 
      LGVVPRCVE FLGKTHNPS LVFEACWAL TNIASGTSE 
      QTAAVVQEG ALPKLQQLL SSNRIDVVE QAIWAIGNI 
      AGDGPESRD LVLNYGVLP SLINLIKPN TTLSLLRNT 
      VWVISNLCR NKNPYPDFE LVKPALPVL ARLLSHDDK 
      DVLADTCWA LSYLTDGSN EKIQAVLDT GLIDRLVML 
      LYSEESTVL TPALRAVGN IVTGNDHQT DMVINAGAL 
      DCMIRLLQH SRLNIVKEA AWTVSNITA GNSEQIQKV 
      LDAGILPYL LHVLQTGDF KSQKEAAWA VTNFTSGGN 
      SAQLAQLVE MGALKPMCN LLNSKDSKT IIVVLEGLN 
      NILSAAAKL NQAEKVAIM IEECGGLDG IEALQSHDN 
      EKVYERALH IIENYFADG EEGVTVTVA NGEIQFATP 
      NMADQQQSP FSF
    CDS sequence
    • >TC000505 RA
      atgggagac gagaacagg atccgttcg tttaaaaac 
      aagggaaaa gactgtgaa gaaatgcgc cgccgacgg 
      gccggccag accatcgaa ctgcggaaa gcaaaaaag 
      gaagaccag ttgttgaag aggcgcaac atatcgttg 
      caagaagag gcctcaact ccccttcag gagaacaac 
      tccgtgtcg ctgatcacc atgtctgcc gaagaaatc 
      ttgatggga atgatgagc cctgacgag aacctccag 
      tttaaggcc acccaagcc tgccgcaaa atgctcagc 
      cgcgagcgg aacccccca atcgaccac atgatccgc 
      ttgggggtt gtcccgcga tgcgtcgag ttcctgggc 
      aagacccac aacccatcg ctggtgttt gaggcttgc 
      tgggccctc acgaacatc gcgtctggc acttccgag 
      cagaccgca gccgtggtc caggagggc gcccttcct 
      aagctccaa cagctgctc tcctcaaac cgcatcgac 
      gtcgtggag caggccatc tgggcgatt ggtaacatt 
      gcaggtgat gggcccgag agccgcgat ttggtgctc 
      aactacggc gttttgccc agtcttatc aacttaatc 
      aaaccaaac actaccttg tccttgtta cgcaatact 
      gtgtgggtt atttctaat ctttgccgc aataaaaat 
      ccgtacccc gattttgag ttagtcaag ccggcttta 
      cccgtcttg gcgagactt ttgtcgcat gacgataag 
      gatgttttg gccgacacg tgctgggct ttgagttat 
      ttaaccgat ggtagcaat gagaaaatt caagcggtg 
      cttgacacc ggtttaatt gaccgccta gttatgctt 
      ttgtatagt gaggaaagt accgtccta actccggcc 
      ttgagggcc gttggtaac attgtgacc ggaaacgac 
      catcagact gatatggtg atcaatgcc ggggcttta 
      gattgtatg atccgttta ttgcaacac agtagactg 
      aacattgtc aaagaagct gcctggaca gtttcaaac 
      attactgcc ggaaatagt gagcaaatc caaaaagtc 
      ctagatgcc gggattttg ccttatctt cttcacgtc 
      ctacaaacc ggcgacttt aaatcgcaa aaagaggca 
      gcttgggct gttactaat ttcacaagt ggcgggaat 
      tccgcccaa ttggcgcaa cttgtggaa atgggggcg 
      ttgaaaccc atgtgtaat ctcctgaat tccaaagat 
      agcaagact attatcgtg gtgttggag ggattgaat 
      aacatttta tcggccgct gccaagtta aatcaagcc 
      gagaaagtg gccataatg attgaggag tgtggcggt 
      ttggacggt attgaggcc ttgcaaagt cacgacaat 
      gagaaagtc tacgaacgg gctttgcac attatcgag 
      aattatttc gccgatggt gaggaggga gtcactgtt 
      actgtagca aatggggag attcagttc gctactcct 
      aatatggcc gatcaacaa caatcaccc ttttccttc 
      taa
    mRNA sequence
    • >TC000505 RA
      aaataattc tccaaattt gataattct attggccaa 
      tttggtggg actgctttt ctattggcc aagtgaagc 
      ttgctcgtt attggctgc ccgtcatta attgtcagt 
      gtgacgttc aggaactgc aacggaaat tttgaccgt 
      agaattttt gtcgtttta ttttttaat cggttgtgg 
      agactattc actaatagt tttaatagt tttggtgcc 
      tagtttaaa tataataga aaaatggga gacgagaac 
      aggatccgt tcgtttaaa aacaaggga aaagactgt 
      gaagaaatg cgccgccga cgggccggc cagaccatc 
      gaactgcgg aaagcaaaa aaggaagac cagttgttg 
      aagaggcgc aacatatcg ttgcaagaa gaggcctca 
      actcccctt caggagaac aactccgtg tcgctgatc 
      accatgtct gccgaagaa atcttgatg ggaatgatg 
      agccctgac gagaacctc cagtttaag gccacccaa 
      gcctgccgc aaaatgctc agccgcgag cggaacccc 
      ccaatcgac cacatgatc cgcttgggg gttgtcccg 
      cgatgcgtc gagttcctg ggcaagacc cacaaccca 
      tcgctggtg tttgaggct tgctgggcc ctcacgaac 
      atcgcgtct ggcacttcc gagcagacc gcagccgtg 
      gtccaggag ggcgccctt cctaagctc caacagctg 
      ctctcctca aaccgcatc gacgtcgtg gagcaggcc 
      atctgggcg attggtaac attgcaggt gatgggccc 
      gagagccgc gatttggtg ctcaactac ggcgttttg 
      cccagtctt atcaactta atcaaacca aacactacc 
      ttgtccttg ttacgcaat actgtgtgg gttatttct 
      aatctttgc cgcaataaa aatccgtac cccgatttt 
      gagttagtc aagccggct ttacccgtc ttggcgaga 
      cttttgtcg catgacgat aaggatgtt ttggccgac 
      acgtgctgg gctttgagt tatttaacc gatggtagc 
      aatgagaaa attcaagcg gtgcttgac accggttta 
      attgaccgc ctagttatg cttttgtat agtgaggaa 
      agtaccgtc ctaactccg gccttgagg gccgttggt 
      aacattgtg accggaaac gaccatcag actgatatg 
      gtgatcaat gccggggct ttagattgt atgatccgt 
      ttattgcaa cacagtaga ctgaacatt gtcaaagaa 
      gctgcctgg acagtttca aacattact gccggaaat 
      agtgagcaa atccaaaaa gtcctagat gccgggatt 
      ttgccttat cttcttcac gtcctacaa accggcgac 
      tttaaatcg caaaaagag gcagcttgg gctgttact 
      aatttcaca agtggcggg aattccgcc caattggcg 
      caacttgtg gaaatgggg gcgttgaaa cccatgtgt 
      aatctcctg aattccaaa gatagcaag actattatc 
      gtggtgttg gagggattg aataacatt ttatcggcc 
      gctgccaag ttaaatcaa gccgagaaa gtggccata 
      atgattgag gagtgtggc ggtttggac ggtattgag 
      gccttgcaa agtcacgac aatgagaaa gtctacgaa 
      cgggctttg cacattatc gagaattat ttcgccgat 
      ggtgaggag ggagtcact gttactgta gcaaatggg 
      gagattcag ttcgctact cctaatatg gccgatcaa 
      caacaatca cccttttcc ttctaagtt tcaaccgat 
      gcgtttagt tcattcctt attgtgtta gtgacacgt 
      tttgtattt ttatagtct gtattacga tatgttccc 
      agttttcga aataaaaat tatgttaaa cc

    If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
    Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.

    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY